Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as an N-terminal His10-tagged protein | Chloroflexus aurantiacus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydroxylamine | - |
Chloroflexus aurantiacus | |
additional information | sodium borohydride has no effect | Chloroflexus aurantiacus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
2 * 47000 | Chloroflexus aurantiacus |
73000 | - |
gel filtration | Chloroflexus aurantiacus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylfumaryl-CoA | Chloroflexus aurantiacus | the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation | 3-methylfumaryl-CoA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chloroflexus aurantiacus | A9WC36 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Chloroflexus aurantiacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylfumaryl-CoA | the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation | Chloroflexus aurantiacus | 3-methylfumaryl-CoA | - |
r | |
2-methylfumaryl-CoA | 2-methylfumaryl-CoA i.e. (E)-3-carboxy-2-methylprop-2-enoyl-CoA. The enzyme acts as an intramolecular CoA transferase and does not transfer CoA to free mesaconate | Chloroflexus aurantiacus | 3-methylfumaryl-CoA | 3-methylfumaryl-CoA i.e. (E)-3-carboxybut-2-enoyl-CoA | r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 47000 | Chloroflexus aurantiacus |
Synonyms | Comment | Organism |
---|---|---|
MCT | - |
Chloroflexus aurantiacus |
mesaconyl-CoA C1-C4 CoA transferase | - |
Chloroflexus aurantiacus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Chloroflexus aurantiacus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
840 | - |
2-methylfumaryl-CoA | 55°C, pH not specified in the publication | Chloroflexus aurantiacus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 7.8 | at 20°C | Chloroflexus aurantiacus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
at 20°C, 50% of maximal activity | Chloroflexus aurantiacus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation | Chloroflexus aurantiacus |