Any feedback?
Literature summary for 5.3.99.7 extracted from
- Heterologous production of different styrene oxide isomerases for the highly efficient synthesis of phenylacetaldehyde (2017), J. Biotechnol., 252, 43-49 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pseudomonas fluorescens |
| expressed in Escherichia coli BL21(DE3) cells | Rhodococcus opacus |
| expressed in Escherichia coli BL21(DE3) cells | Sphingopyxis fribergensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| styrene oxide | Pseudomonas fluorescens | - |
phenylacetaldehyde | - |
? |  |
| styrene oxide | Rhodococcus opacus | - |
phenylacetaldehyde | - |
? | |
| styrene oxide | Sphingopyxis fribergensis | - |
phenylacetaldehyde | - |
? | |
| styrene oxide | Rhodococcus opacus 1CP | - |
phenylacetaldehyde | - |
? | |
| styrene oxide | Pseudomonas fluorescens ST | - |
phenylacetaldehyde | - |
? | |
| styrene oxide | Sphingopyxis fribergensis Kp5.2 | - |
phenylacetaldehyde | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | O06836 | - |
- |
| Pseudomonas fluorescens ST | O06836 | - |
- |
| Rhodococcus opacus | A0A076JZU4 | - |
- |
| Rhodococcus opacus 1CP | A0A076JZU4 | - |
- |
| Sphingopyxis fribergensis | A0A076K0M9 | - |
- |
| Sphingopyxis fribergensis Kp5.2 | A0A076K0M9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial purification | Pseudomonas fluorescens |
| partial purification | Rhodococcus opacus |
| partial purification | Sphingopyxis fribergensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.1 | - |
crude cell extract, at pH 7.2 and 37°C | Pseudomonas fluorescens |
| 9.79 | - |
after 2.4fold purification, at pH 7.2 and 37°C | Pseudomonas fluorescens |
| 32.2 | - |
crude cell extract, at pH 7.2 and 37°C | Sphingopyxis fribergensis |
| 75.9 | - |
crude cell extract, at pH 7.2 and 37°C | Rhodococcus opacus |
| 89.7 | - |
after 2.8fold purification, at pH 7.2 and 37°C | Sphingopyxis fribergensis |
| 150 | - |
after 2fold purification, at pH 7.2 and 37°C | Rhodococcus opacus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| styrene oxide | - |
Pseudomonas fluorescens | phenylacetaldehyde | - |
? | |
| styrene oxide | - |
Rhodococcus opacus | phenylacetaldehyde | - |
? | |
| styrene oxide | - |
Sphingopyxis fribergensis | phenylacetaldehyde | - |
? | |
| styrene oxide | - |
Rhodococcus opacus 1CP | phenylacetaldehyde | - |
? | |
| styrene oxide | - |
Pseudomonas fluorescens ST | phenylacetaldehyde | - |
? | |
| styrene oxide | - |
Sphingopyxis fribergensis Kp5.2 | phenylacetaldehyde | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SOI | - |
Pseudomonas fluorescens |
| SOI | - |
Rhodococcus opacus |
| SOI | - |
Sphingopyxis fribergensis |
| StyC | - |
Pseudomonas fluorescens |
| StyC | - |
Rhodococcus opacus |
| StyC | - |
Sphingopyxis fribergensis |
| styrene oxide isomerase | - |
Pseudomonas fluorescens |
| styrene oxide isomerase | - |
Rhodococcus opacus |
| styrene oxide isomerase | - |
Sphingopyxis fribergensis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
