Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant C-terminally His4-tagged enzyme replacing the first 28 amino acid residues in the N-terminal transmembrane domain with the hydrophilic sequence MAKKTSS for TXAS cDNA in Escherichia coli strain BL21/(DE3)pLys | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
W133F | site-directed mutagenesis, ligand binding analysis compared to the wild-type enzyme | Homo sapiens |
W203F | site-directed mutagenesis, ligand binding analysis compared to the wild-type enzyme | Homo sapiens |
W31F | site-directed mutagenesis, ligand binding analysis compared to the wild-type enzyme | Homo sapiens |
W31F/W65F/W133F/W203F/W466F | site-directed mutagenesis, ligand binding analysis compared to the wild-type enzyme | Homo sapiens |
W466F | site-directed mutagenesis, ligand binding analysis compared to the wild-type enzyme | Homo sapiens |
W65F | site-directed mutagenesis, ligand binding analysis compared to the wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
clotrimazole | as a heme ligand, molecular dynamics simulations of clotrimazole and 2-p-toluidinylnaphthalene-6-sulfonic acid-docking enzyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | thromboxane A2 synthase catalyzes an isomerization of prostaglandins H2 to give rise to thromboxane A2 along with a fragmentation reaction to 12-L-hydroxy-5,8,10-heptadecatrienoic acid and malondialdehyde. 2-p-Toluidinylnaphthalene-6-sulfonic acid is utilized as a probe to assess the spatial relationship and binding dynamics of ligand-enzyme interactions by steady-state and time-resolved fluorescence spectroscopy | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant C-terminally His4-tagged modified enzyme from Escherichia coli strain BL21/(DE3)pLys by nickel affinity and hydroxyapatite chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | thromboxane A2 synthase catalyzes an isomerization of prostaglandins H2 to give rise to thromboxane A2 along with a fragmentation reaction to 12-L-hydroxy-5,8,10-heptadecatrienoic acid and malondialdehyde. 2-p-Toluidinylnaphthalene-6-sulfonic acid is utilized as a probe to assess the spatial relationship and binding dynamics of ligand-enzyme interactions by steady-state and time-resolved fluorescence spectroscopy | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme structure molecular homology modeling using the crystal structure of human microsomal CYP3A4, PDB entry 1TQN, as the template structure | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
thromboxane A2 synthase | - |
Homo sapiens |
thromboxane synthase | - |
Homo sapiens |
TXAS | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | bound at the active site | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | the adaptive active site in the enzyme might be capable of binding multiple ligands simultaneously because of its large size and flexibility. Ligand and substrate binding structure, competitive binding experiments with Nile Red, a fluorescence dye, and 2-p-toluidinylnaphthalene-6-sulfonic acid, and the computer-simulated TXAS structure with clotrimazole as a heme ligand, molecular dynamics simulations of clotrimazole and 2-p-toluidinylnaphthalene-6-sulfonic acid-docking enzyme, overview | Homo sapiens |