BRENDA - Enzyme Database show
show all sequences of 5.3.99.4

Purification and characterization of recombinant human prostacyclin synthase

Wada, M.; Yokoyama, C.; Hatae, T.; Shimonishi, M.; Nakamura, M.; Imai, Y.; Ullrich, V.; Tanabe, T.; J. Biochem. 135, 455-463 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Spodoptera frugiperda 21 cells
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
C441H
no heme-binding ability, no activity
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
Tranylcypromine
-
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
prostaglandin H2
at 24°C
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
localization of recombinant enzyme in infected Sf21 cells
Homo sapiens
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48000
-
mutant C441H, SDS-PAGE; wild-type, SDS-PAGE
Homo sapiens
57100
-
calculated from the primary structure, deduced from the cDNA sequence
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
prostaglandin H2
Homo sapiens
-
prostacyclin
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Oxidation Stability
Oxidation Stability
Organism
human PGIS is significantly unstable when the heme cofactor appears in the reduction and/or reduction-CO binding state
Homo sapiens
Purification (Commentary)
Commentary
Organism
recombinant enzyme is purified to homogeneity
Homo sapiens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.6
-
solubilized microsomes
Homo sapiens
2.6
-
after calcium phosphate gel
Homo sapiens
4.3
-
after DEAE-sepharose chromatography
Homo sapiens
7.1
-
after hydroxyapatite chromatography
Homo sapiens
15
-
purified enzyme, at 24°C
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
prostaglandin H2
-
662059
Homo sapiens
prostacyclin
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
heme
-
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Spodoptera frugiperda 21 cells
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
heme
-
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C441H
no heme-binding ability, no activity
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Tranylcypromine
-
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
prostaglandin H2
at 24°C
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
localization of recombinant enzyme in infected Sf21 cells
Homo sapiens
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48000
-
mutant C441H, SDS-PAGE; wild-type, SDS-PAGE
Homo sapiens
57100
-
calculated from the primary structure, deduced from the cDNA sequence
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
prostaglandin H2
Homo sapiens
-
prostacyclin
-
-
?
Oxidation Stability (protein specific)
Oxidation Stability
Organism
human PGIS is significantly unstable when the heme cofactor appears in the reduction and/or reduction-CO binding state
Homo sapiens
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme is purified to homogeneity
Homo sapiens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.6
-
solubilized microsomes
Homo sapiens
2.6
-
after calcium phosphate gel
Homo sapiens
4.3
-
after DEAE-sepharose chromatography
Homo sapiens
7.1
-
after hydroxyapatite chromatography
Homo sapiens
15
-
purified enzyme, at 24°C
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
prostaglandin H2
-
662059
Homo sapiens
prostacyclin
-
-
-
?
Other publictions for EC 5.3.99.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748245
Tsai
Improving the regenerative po ...
Rattus norvegicus
J. Biomed. Sci.
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34
2017
-
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-
-
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2
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1
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-
-
-
-
-
-
748466
Yang
Homology modeling and molecul ...
Homo sapiens
J. Phys. Chem. B
121
11229-11240
2017
-
-
-
1
-
-
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-
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1
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1
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1
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1
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1
1
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1
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1
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-
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-
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749211
Sasaki
Role of prostacyclin synthase ...
Homo sapiens
Prostaglandins Other Lipid Mediat.
133
49-52
2017
-
-
-
-
-
-
-
-
-
-
-
1
-
1
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2
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1
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1
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2
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1
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-
-
-
-
-
-
-
1
1
1
1
-
-
749359
Velez Edwards
Nonsteroidal anti-inflammator ...
Homo sapiens
Sci. Rep.
7
9874
2017
-
-
-
-
-
-
-
-
-
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1
-
2
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
748449
Camacho
Prostacyclin-synthase express ...
Homo sapiens
J. Pathol.
235
125-135
2015
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
3
-
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1
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3
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
748544
Klein
Prostacyclin synthase upregul ...
Homo sapiens
Mediators Inflamm.
2015
654151
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
7
-
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7
-
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-
-
-
-
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-
-
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-
1
-
-
1
-
-
715791
Galan
Angiotensin II differentially ...
Rattus norvegicus
J. Hypertens.
29
529-536
2011
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
4
-
-
1
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1
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1
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1
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4
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
716080
Lian
In vivo therapy of myocardial ...
Homo sapiens
Life Sci.
88
455-464
2011
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
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-
3
-
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1
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1
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1
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3
-
-
1
-
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-
-
-
-
-
-
-
-
1
1
-
-
-
727666
Chao
Probing the interaction betwee ...
Danio rerio, Homo sapiens
J. Am. Chem. Soc.
133
18870-18879
2011
-
-
2
-
-
-
6
-
-
-
-
-
-
4
-
-
2
-
-
-
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2
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2
2
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6
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2
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-
-
2
2
-
-
-
701619
He
Enhanced tyrosine nitration of ...
Homo sapiens
Am. J. Pathol.
176
2542-2549
2010
-
1
-
-
-
-
-
-
-
-
-
1
-
5
-
1
-
-
-
3
-
-
1
-
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1
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1
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1
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
701992
Cyrus
Expression of thromboxane synt ...
Mus musculus
Atherosclerosis
208
376-381
2010
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
1
-
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1
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-
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1
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1
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1
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2
-
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2
-
-
702474
Cathcart
The role of prostacyclin synth ...
Homo sapiens
Biochim. Biophys. Acta
1805
153-166
2010
3
1
1
-
-
-
1
-
3
-
1
1
-
2
-
-
1
-
-
6
-
-
1
-
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-
-
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-
3
1
1
-
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1
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3
-
1
1
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-
1
-
6
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
704650
Gurgul-Convey
Protection against cytokine to ...
Homo sapiens
J. Biol. Chem.
285
11121-11128
2010
-
1
1
-
-
-
1
-
-
-
1
1
-
1
-
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1
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1
1
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1
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1
1
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1
-
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-
-
-
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-
-
705476
Keith
Chemoprevention of murine lung ...
Mus musculus
Lung Cancer
70
37-42
2010
-
1
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
2
-
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1
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1
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1
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2
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1
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1
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1
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-
701168
Gillio-Meina
Expression patterns and role o ...
Rattus norvegicus
Reproduction
137
537-552
2009
-
-
-
-
-
-
-
-
3
-
1
1
-
2
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3
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1
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3
-
1
1
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3
-
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1
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701991
Ishii
Mesenchymal stem cell-based ge ...
Rattus norvegicus
Atherosclerosis
206
109-118
2009
-
1
1
-
-
-
-
-
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1
1
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3
-
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1
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1
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702350
Yeh
Characterization of the peroxi ...
Homo sapiens
Biochemistry
48
917-928
2009
-
-
1
-
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-
-
1
-
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1
-
2
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1
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1
1
1
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1
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1
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1
-
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1
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1
-
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-
1
1
1
-
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-
1
-
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-
-
-
-
-
-
-
705205
Hein
C-reactive protein impairs cor ...
Sus scrofa
J. Mol. Cell. Cardiol.
47
196-202
2009
-
1
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2
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1
1
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2
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1
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1
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1
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2
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1
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1
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680910
Li
Structures of prostacyclin syn ...
Danio rerio
J. Biol. Chem.
283
2917-2926
2008
-
-
1
1
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-
1
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1
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1
1
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1
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1
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1
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1
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-
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-
693452
Lee
Human follicular dendritic cel ...
Homo sapiens
J. Immunol.
180
1390-1397
2008
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
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1
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1
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-
693514
Camacho
Retinoic acid induces PGI synt ...
Homo sapiens
J. Lipid Res.
49
1707-1714
2008
1
-
-
-
-
-
-
-
-
-
-
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2
-
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2
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1
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2
-
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-
694938
Pakrasi
Cyclooxygenase-2-derived endog ...
Mus musculus
Prostaglandins Leukot. Essent. Fatty Acids
79
27-33
2008
-
1
-
-
-
-
1
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1
-
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-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
677494
Wang
Prostacyclin in endotoxemia-in ...
Rattus norvegicus
Am. J. Physiol. Renal Physiol.
293
F1131-F1136
2007
-
1
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677820
Yeh
Reaction mechanisms of 15-hydr ...
Homo sapiens
Arch. Biochem. Biophys.
461
159-168
2007
-
-
1
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
678436
Kawka
Double-label expression studie ...
Canis lupus familiaris, Homo sapiens, Rattus norvegicus
Biochim. Biophys. Acta
1771
45-54
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
7
-
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-
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-
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-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681029
Griffoni
Selective inhibition of prosta ...
Bos taurus, Homo sapiens
J. Cell. Mol. Med.
11
327-338
2007
-
-
-
-
-
-
2
-
1
-
-
-
-
4
-
-
-
-
-
3
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-
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-
-
-
-
-
-
-
-
-
-
-
681106
Kengni
Regulation of prostaglandin D ...
Rattus norvegicus
J. Endocrinol.
195
301-311
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
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2
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
662509
Fang
Induction of prostacyclin/PGI( ...
Rattus norvegicus
J. Cereb. Blood Flow Metab.
26
491-501
2006
-
1
1
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
1
-
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1
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1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
678166
Ruan
Engineering of a protein with ...
Homo sapiens
Biochemistry
45
14003-14011
2006
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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1
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-
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1
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-
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-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
679011
Poole
Prostacyclin synthase and arac ...
Homo sapiens
Cancer Epidemiol. Biomarkers Prev.
15
502-508
2006
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
679435
Nie
Endothelial nitric oxide synth ...
Bos taurus, Mus musculus
Diabetes
55
3133-3141
2006
-
2
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
2
-
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-
-
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-
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660808
Ruan
The N-terminal membrane anchor ...
Homo sapiens
Arch. Biochem. Biophys.
435
372-381
2005
-
-
1
-
2
-
-
-
1
-
1
1
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
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-
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1
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1
-
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-
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-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660924
Wu
Cellular and molecular biology ...
Bos taurus, Homo sapiens, Mus musculus
Biochem. Biophys. Res. Commun.
338
45-52
2005
1
1
1
-
-
-
4
-
3
-
3
3
-
3
-
-
2
-
-
14
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
1
1
1
3
-
-
-
-
4
-
-
3
-
3
3
-
-
-
2
-
14
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661260
Yeh
Characterization of heme envir ...
Homo sapiens
Biochim. Biophys. Acta
1738
121-132
2005
-
-
1
-
-
-
-
1
1
-
-
1
-
3
-
-
1
-
-
-
1
-
3
-
-
-
-
-
-
-
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1
-
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1
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-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
662795
Kajita
Role of COX-2, thromboxane A2 ...
Homo sapiens
Mod. Pathol.
18
221-227
2005
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661425
Keith
Pulmonary prostacyclin synthas ...
Rattus norvegicus
Cancer Res.
64
5897-5904
2004
-
1
1
-
-
-
-
-
-
-
-
1
-
6
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
662059
Wada
Purification and characterizat ...
Homo sapiens
J. Biochem.
135
455-463
2004
-
-
1
-
1
-
1
1
1
-
2
1
-
3
1
-
1
-
-
-
5
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
1
-
1
1
-
2
1
-
1
-
1
-
-
5
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649730
Deng
Substrate access channel topol ...
Homo sapiens
Biochem. J.
362
545-551
2002
-
-
1
-
-
-
-
-
1
-
1
1
-
2
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
37476
Ullrich
Properties of prostacyclin syn ...
Bos taurus
Eicosanoids and other Bioactive Lipids in Cancer, Inflammation, and Radiation Injury, (Honn, K. V. et al. , eds. ) Plenum Press New York
2
113-119
1997
-
-
-
-
-
-
1
-
1
-
-
-
-
4
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
37483
Zou
Tyrosine nitration as a mechan ...
Homo sapiens
Biol. Chem.
378
707-713
1997
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
37485
Wade
Nitric oxide modulates the act ...
Bos taurus
Arch. Biochem. Biophys.
347
174-180
1997
1
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
37478
Zou
Peroxynitrite formed by simult ...
Bos taurus
FEBS Lett.
382
101-104
1996
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
37475
Tanabe
Prostacyclin and thromboxane s ...
Homo sapiens, Sus scrofa
J. Lipid Mediat. Cell Signal.
12
243-255
1995
-
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
2
-
2
-
-
4
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
37477
Wade
-
'Suicide' inactivation of pros ...
Bos taurus
Arch. Biochem. Biophys.
321
435-458
1995
-
-
-
-
-
-
2
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
37472
Hara
Isolation and molecular clonin ...
Bos taurus
J. Biol. Chem.
269
19897-19903
1994
-
-
1
-
-
-
-
3
-
-
2
1
-
3
-
-
-
-
-
2
1
-
4
1
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
2
1
-
-
-
-
-
2
1
-
4
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
37474
Ullrich
-
Prostacyclin- und Thromboxan-S ...
Bos taurus, Sus scrofa
Angew. Chem.
106
1987-1996
1994
-
-
-
-
-
-
4
-
2
-
3
-
-
2
-
-
-
2
-
-
-
-
10
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
2
-
3
-
-
-
-
-
-
-
-
-
10
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
37480
Pereira
Molecular cloning and characte ...
Bos taurus
Biochem. Biophys. Res. Commun.
203
59-66
1994
-
-
1
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
37479
Pereira
Bovine prostacyclin synthase: ...
Bos taurus
Biochem. Biophys. Res. Commun.
197
1041-1048
1993
-
-
-
-
-
-
1
-
-
-
1
-
-
2
-
-
1
-
-
2
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
1
-
2
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3200
Haurand
-
Thromboxane synthase and prost ...
Bos taurus, Sus scrofa
Front. Biotransform.
6
183-199
1992
-
-
-
-
-
-
3
-
2
-
-
-
-
2
-
-
-
2
-
2
-
-
6
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
2
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
37481
McLennan
Prostaglandin synthetase and p ...
Rattus norvegicus
J. Anat.
178
243-253
1991
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
37482
Chegini
Cellular distribution of prost ...
Bos taurus
Mol. Cell. Endocrinol.
71
133-140
1990
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
37473
Lin
Characterization of the second ...
Homo sapiens
Arch. Biochem. Biophys.
352
78-84
1988
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
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-
3127
McNamara
Modulation of prostacyclin syn ...
Bos taurus, Canis lupus familiaris, Homo sapiens, Ovis aries
Biochem. Biophys. Res. Commun.
118
33-39
1984
2
-
-
-
-
-
1
-
2
-
-
-
-
8
1
-
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4
-
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-
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-
-
-
-
-
-
-
-
-
-
37468
DeWitt
Purification of prostacyclin s ...
Bos taurus
J. Biol. Chem.
258
3285-3293
1983
-
-
-
-
-
-
3
-
1
-
1
-
-
2
-
-
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-
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-
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-
-
-
-
-
37469
Smith
Purification, quantitation, an ...
Bos taurus
Adv. Prostaglandin Thromboxane Leukot. Res.
11
87-92
1983
-
1
-
-
-
-
4
-
2
-
1
-
-
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-
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3
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-
37470
Graf
-
Die Prostacyclin-Synthase, ein ...
Sus scrofa
Angew. Chem.
95
497-498
1983
-
-
-
-
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-
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1
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-
37464
Salmon
Preparation and assay of prost ...
Sus scrofa
Methods Enzymol.
86
91-99
1982
-
-
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-
1
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-
-
-
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1
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-
37465
DeWitt
Monoclonal antibodies against ...
Bos taurus
Methods Enzymol.
86
240-246
1982
-
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1
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-
37466
Tai
Assay of prostacyclin synthase ...
Sus scrofa
Methods Enzymol.
86
99-105
1982
-
-
-
-
-
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-
1
-
-
-
-
1
-
-
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-
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-
-
-
-
-
-
-
-
-
-
37467
Graf
-
Prostacyclin synthase as a cyt ...
Sus scrofa
Dev. Biochem.
23
103-106
1982
-
-
-
-
-
-
-
-
1
-
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-
-
1
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
37463
Ullrich
Spectral evidence for the cyto ...
Sus scrofa
Biochem. Pharmacol.
30
2033-2036
1981
-
-
-
-
-
-
2
-
1
-
-
-
-
1
-
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-
-
-
-
-
-
-
-
-
-
-
37461
Turk
Inactivation of vascular prost ...
Bos taurus
Biochem. Biophys. Res. Commun.
95
1628-1634
1980
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
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-
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-
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-
-
-
-
-
-
-
-
-
-
-
37462
Beitz
Influence of human low density ...
Sus scrofa
Biochim. Biophys. Acta
620
352-355
1980
2
-
-
-
-
-
1
-
1
-
-
-
-
3
-
-
-
-
-
5
-
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-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
37458
Wlodawer
Some properties of prostacycli ...
Sus scrofa
FEBS Lett.
97
32-36
1979
-
-
-
-
-
1
1
-
1
-
-
-
-
2
-
-
-
-
-
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-
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-
1
-
-
-
-
-
-
-
-
-
-
-
37459
Ham
Peroxidase-dependent deactivat ...
Ovis aries
J. Biol. Chem.
254
2191-2194
1979
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
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-
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-
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1
1
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-
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-
-
-
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-
-
-
-
-
37460
Terashita
A specific prostaglandin I2 sy ...
Bos taurus
Biochem. Biophys. Res. Commun.
91
72-78
1979
-
-
-
-
-
-
5
-
1
-
-
-
-
1
-
-
-
-
-
1
-
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1
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-
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