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Literature summary for 5.3.99.4 extracted from

  • Wada, M.; Yokoyama, C.; Hatae, T.; Shimonishi, M.; Nakamura, M.; Imai, Y.; Ullrich, V.; Tanabe, T.
    Purification and characterization of recombinant human prostacyclin synthase (2004), J. Biochem., 135, 455-463.
    View publication on PubMed
Search for more literature for 5.3.99.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Spodoptera frugiperda 21 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C441H no heme-binding ability, no activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
tranylcypromine
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 prostaglandin H2 at 24°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome localization of recombinant enzyme in infected Sf21 cells Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 wild-type, SDS-PAGE Homo sapiens
48000
-
 mutant C441H, SDS-PAGE Homo sapiens
57100
-
 calculated from the primary structure, deduced from the cDNA sequence Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
prostaglandin H2 Homo sapiens
-
 prostacyclin
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Oxidation Stability

Oxidation Stability Organism
human PGIS is significantly unstable when the heme cofactor appears in the reduction and/or reduction-CO binding state Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme is purified to homogeneity Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6
-
 solubilized microsomes Homo sapiens
2.6
-
 after calcium phosphate gel Homo sapiens
4.3
-
 after DEAE-sepharose chromatography Homo sapiens
7.1
-
 after hydroxyapatite chromatography Homo sapiens
15
-
 purified enzyme, at 24°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
prostaglandin H2
-
 Homo sapiens prostacyclin
-
 ?

Synonyms

Synonyms Comment Organism
PGIS
-
 Homo sapiens
prostacyclin synthase
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
heme
-
 Homo sapiens