Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.99.3 extracted from

  • Brock, J.S.; Hamberg, M.; Balagunaseelan, N.; Goodman, M.; Morgenstern, R.; Strandback, E.; Samuelsson, B.; Rinaldo-Matthis, A.; Haeggstroem, J.Z.
    A dynamic Asp-Arg interaction is essential for catalysis in microsomal prostaglandin E2 synthase (2016), Proc. Natl. Acad. Sci. USA, 113, 972-977 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.99.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Homo sapiens

Protein Variants

Protein Variants Comment Organism
D49N the mutant shows severely reduced activity compared to the wild type enzyme Homo sapiens
R126K the mutant shows severely reduced activity compared to the wild type enzyme Homo sapiens
R126Q the mutant shows severely reduced activity compared to the wild type enzyme Homo sapiens
S127A the mutant shows about wild type activity Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Prostaglandin H2 Homo sapiens
-
 Prostaglandin E2
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O14684
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-affinity column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Prostaglandin H2
-
 Homo sapiens Prostaglandin E2
-
 ?

Synonyms

Synonyms Comment Organism
microsomal prostaglandin E2 synthase type 1
-
 Homo sapiens
mPGES-1
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
glutathione dependent on Homo sapiens