Literature summary for 5.3.99.3 extracted from

Jegerschoeld, C.; Pawelzik, S.C.; Purhonen, P.; Bhakat, P.; Gheorghe, K.R.; Gyobu, N.; Mitsuoka, K.; Morgenstern, R.; Jakobsson, P.J.; Hebert, H.
Structural basis for induced formation of the inflammatory mediator prostaglandin E2 (2008), Proc. Natl. Acad. Sci. USA, 105, 11110-11115.

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Application

Application	Comment	Organism
medicine	MPGES1 has been implicated in a number of human diseases or pathological conditions, such as rheumatoid arthritis, fever, and pain, and is therefore regarded as a primary target for development of novel antiinflammatory drugs	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
the structure of human MPGES1 is determined in complex with the tripeptide gamma-L-glutamyl-L-cysteinyl-glycine, glutathione, at 3.5 A in-plane resolution using electron crystallography	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
E66A	mutant, fraction of wild-type activity, 53%	Homo sapiens
E77A	mutant, no activity	Homo sapiens
H72A	mutant, fraction of wild-type activity, 32%	Homo sapiens
R110A	mutant, no activity	Homo sapiens
R110S	mutant, no activity	Homo sapiens
R67A	mutant, no activity	Homo sapiens
R70A	mutant, fraction of wild-type activity, 58%	Homo sapiens
R70A/Y117A	mutant, fraction of wild-type activity, 7%	Homo sapiens
R70S	mutant, full activity	Homo sapiens
Y117A	mutant, fraction of wild-type activity, 1%	Homo sapiens
Y117F	mutant, full activity	Homo sapiens
Y130I	mutant, fraction of wild-type activity, 15%	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Homo sapiens	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate	Homo sapiens	-	(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O14684	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate	-	Homo sapiens	(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate	-	?

Subunits

Subunits	Comment	Organism
homotrimer	-	Homo sapiens

Synonyms

Synonyms	Comment	Organism
microsomal prostaglandin E synthase-1	-	Homo sapiens
MPGES1	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
glutathione	-	Homo sapiens

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Release 2023.1 (January 2023)