Literature summary for 5.3.99.3 extracted from

Yamada, T.; Komoto, J.; Watanabe, K.; Ohmiya, Y.; Takusagawa, F.
Crystal structure and possible catalytic mechanism of microsomal prostaglandin E synthase type 2 (mPGES-2) (2005), J. Mol. Biol., 348, 1163-1176.

Search for more literature for 5.3.99.3

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Macaca fascicularis
overexpression of truncated mPGES-2, comprising residues 1-87, with an extended His-tag in Escherichia coli strain BL21 by nickel affinity chromatography	Macaca fascicularis

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His-tagged truncated mPGES-2, complexed with anti-inflammatory drug IMN, 10 mg/ml protein in 1.7 M ammonium sulfate, 0.1 M sodium acetate-HCl, pH 5.5, and 1.5 mM indomethacin, sitting drop vapour diffusion method, 3 days, 22°C, X-ray diffraction structure determinationand analysis at 2.6 A resolution, modeling	Macaca fascicularis
with an anti-inflammatory drug indomethacin, 2.6 A resolution	Macaca fascicularis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Macaca fascicularis	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
Prostaglandin H2	Macaca fascicularis	-	Prostaglandin E2	-	?

Organism

Organism	UniProt	Comment	Textmining
Macaca fascicularis	Q9N0A4	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Macaca fascicularis
recombinant His-tagged truncated mPGES-2 from Escherichia coli strain BL21 by nickel affinity chromatography	Macaca fascicularis

Reaction

Reaction	Comment	Organism	Reaction ID
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate	catalytic mechanism of mPGES-2, hydrogen bond-forming residues Y107, C113, C110, and F112 are involved, substrate binding and catalytic sites	Macaca fascicularis	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Prostaglandin H2	-	Macaca fascicularis	Prostaglandin E2	-	?

Subunits

Subunits	Comment	Organism
dimer	crystal structure	Macaca fascicularis
More	structure-function analysis, overview	Macaca fascicularis

Synonyms

Synonyms	Comment	Organism
microsomal prostaglandin E synthase type 2	-	Macaca fascicularis
mPGES-2	-	Macaca fascicularis
prostaglandin E synthase type 2	-	Macaca fascicularis

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Release 2023.1 (January 2023)