Cloned (Comment) | Organism |
---|---|
expression of histidine-tagged mPGES-1 in Escherichia coli | Homo sapiens |
General Stability | Organism |
---|---|
activity in solubilized membranes remains stable for a prolonged time in the presence of reduced glutathione and glycerol | Homo sapiens |
resistant to freeze-thawing | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate | - |
Homo sapiens | |
0.16 | - |
prostaglandin G2 | - |
Homo sapiens | |
0.71 | - |
glutathione | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17500 | - |
x * 17500, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged mPGES-1, hydroxyapatite, Ni2+-affinity chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.04 | - |
substrate 15-hydroperoxy-prostaglandin E2 | Homo sapiens |
0.043 | - |
substrate 5-hydroperoxyeicostetraenoic | Homo sapiens |
0.17 | - |
substrate cumene hydroperoxide | Homo sapiens |
0.81 | - |
substrate 1-chloro-2,4-dinitrobenzene | Homo sapiens |
120 | - |
substrate (5Z,13E)-(15S)-9a,11a-epidioxy-15-hydroxyprosta-5,13-dienoate | Homo sapiens |
190 | - |
substrate prostaglandin G2 | Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, 6 months, no loss of activity | Homo sapiens |
4°C, solubilized membranes, 10 d, 50% loss of activity | Homo sapiens |
4°C, solubilized membranes, 24 h, 30% loss of activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5Z,13E)-(15S)-9a,11a-epidioxy-15-hydroxyprosta-5,13-dienoate + GSH | i.e. prostaglandin H2 | Homo sapiens | (5Z,13E)-(15S)-11a,15-dihydroxyprosta-5,13-dienoate + GSSG | i.e. prostaglandin E2 | ? | |
1-chloro-2,4-dinitrobenzene + GSH | glutathione S-transferase activity | Homo sapiens | ? | - |
? | |
15-hydroperoxy-prostaglandin E2 + GSH | - |
Homo sapiens | prostaglandin E2 + GSSG | - |
? | |
5-hydroperoxyeicosatetraenoic acid + 2 GSH | - |
Homo sapiens | 5-hydroxyeicosatetraenoic acid + GSSG + H2O | - |
? | |
cumene hydroperoxide + 2 GSH | - |
Homo sapiens | cumene hydroxide + GSSG + H2O | - |
? | |
prostaglandin G2 + GSH | - |
Homo sapiens | 15-hydroperoxy-prostaglandine E2 + GSSG | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 17500, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
mPGES-1 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21 | - |
glutathione | - |
Homo sapiens | |
50 | - |
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate | - |
Homo sapiens | |
75 | - |
prostaglandine G2 | - |
Homo sapiens |