Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GSH | dependent on | Bos taurus |
General Stability | Organism |
---|---|
thiol compounds, glutathione, dithiothreitol, 2-mercaptoethanol or Cys markedly protect from thermal inactivation | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Bis(3-carboxy-4-nitrophenyl)disulfide | - |
Bos taurus | |
HgCl2 | - |
Bos taurus | |
iodoacetamide | - |
Bos taurus | |
iodoacetic acid | - |
Bos taurus | |
Iodosobenzoic acid | - |
Bos taurus | |
NEM | - |
Bos taurus | |
PCMB | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
Prostaglandin H1 | - |
Bos taurus | |
0.05 | - |
Prostaglandin G1 | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Bos taurus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.6 | - |
prostaglandin H1 | Bos taurus |
1.8 | - |
prostaglandin H2 | Bos taurus |
Storage Stability | Organism |
---|---|
-20°C, 1 mM glutathione, pH 7.0, completely inactivated after a few weeks | Bos taurus |
-70°C, 1 mM glutathione, pH 7.0, stable for at least several months | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Prostaglandin G1 | at 50% of the activity relative to prostaglandin H | Bos taurus | 15-Hydroperoxy-prostaglandin E1 | major product | ? | |
prostaglandin G2 | at 50% of the activity relative to prostaglandin H | Bos taurus | 15-hydroperoxy-prostaglandin E2 | major product | ? | |
Prostaglandin H1 | ir | Bos taurus | Prostaglandin E1 | - |
? | |
Prostaglandin H1 | i.e. 15-hydroxy-9alpha,11alpha-peroxidoprosta-13-enoic acid | Bos taurus | Prostaglandin E1 | - |
? | |
Prostaglandin H2 | i.e. 15-hydroxy-9alpha,11alpha-peroxidoprosta-5,13-dienoic acid | Bos taurus | Prostaglandin E2 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thiol compounds, glutathione, dithiothreitol, 2-mercaptoethanol or cysteine markedly protect from thermal inactivation | Bos taurus |
25 | - |
pH 8.0, 0.1 M potassium phosphate buffer, half-life: 30 min | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | almost inactive below pH 5.5 and above pH 9.5 | Bos taurus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | pH-range of highest stability, 35°C, 7.5 min, either in presence or absence of glutathione | Bos taurus |