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Literature summary for 5.3.99.2 extracted from

  • Kume, S.; Lee, Y.H.; Miyamoto, Y.; Fukada, H.; Goto, Y.; Inui, T.
    Systematic interaction analysis of human lipocalin-type prostaglandin D synthase with small lipophilic ligands (2012), Biochem. J., 446, 279-289.
    View publication on PubMed
Search for more literature for 5.3.99.2

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
C65A/C167A site-directed mutagenesis, the mutant still provides the disulfide bond between Cys89 and Cys186 Homo sapiens
W43F/C65A/C167A site-directed mutagenesis, the W43F mutant cannot be purified owing to the inclusion body formation of protein Homo sapiens
W54F/C65A/W112F/C167A site-directed mutagenesis, the mutant still provides the disulfide bond between Cys89 and Cys186 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information the lipid transporter activity of the enzyme is competitively inhibited by pamitate, stearate and arachnoate Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the N-terminal 22-amino acid residues correspond to the putative secretion signal peptide of the enzyme Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P41222
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) by glutathione affinity chromatography, gel filtration, and dialysis Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
cerebrospinal fluid
-
 Homo sapiens
-

Synonyms

Synonyms Comment Organism
Beta-trace protein
-
 Homo sapiens
L-PGDS
-
 Homo sapiens
lipocalin-type PG D synthase
-
 Homo sapiens
lipocalin-type prostaglandin d synthase
-
 Homo sapiens
Prostaglandin-H2 D-isomerase
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 purified recombinant enzyme, sodium phosphate, pH 7.0, containing 5% v/v ethanol, stable up to Homo sapiens
65 90 purified recombinant enzyme, thermal unfolding in the absence and presence of small lipophilic ligands, two-state unfolding transition, overview Homo sapiens

General Information

General Information Comment Organism
evolution the enzyme belongs to the lipocalin superfamily Homo sapiens
physiological function lipocalin-type prostaglandin D synthase is a multi-functional protein, acting as a prostaglandin D2-producing enzyme and a lipid-transporter. It is involved in the biosynthesis of prostaglandin D2, acting as an endogenous somnogen and allergy response. Binding mechanism of small lipophilic molecules by the enzyme functioning as lipid transporter, e.g. of biliverdin, all-trans-retinoic acid, L-thyroxine (T4), progesterone, and genistein, thermodynamic parameters, overview. The enzme shows broad ligand selectivity for small lipophilic molecules, it binds heme metabolites with significantly higher affinity than other small lipophilic ligands Homo sapiens