Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
macro-seeding using a solution containing 14% polyethylene glycol 6000, 50 mM Tris-HCl, pH 8.4, 5 mM reduced glutathione, 5 mM dithiothreitol, 2.5 mM CaCl2 or MgCl2 and 1% dioxane, structure resolution at 1.8 A | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D93N | not activated by Ca2+ or Mg2+ | Homo sapiens |
D96N | very high basal activity, no activation in the presence of Ca2+ or Mg2+ | Homo sapiens |
D97N | not activated by Ca2+ or Mg2+ | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | increase in activity | Homo sapiens | |
Ca2+ | half maximal effective concentration at 0.4 mM, 1.5fold increase in activity | Homo sapiens | |
Mg2+ | half maximal effective concentration at 0.05 mM, 1.5fold increase in activity, Mg2+ increases the affinity for glutathione | Homo sapiens | |
Sr2+ | increase in activity | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate + glutathione | Homo sapiens | - |
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + glutathione | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O60760 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant PDGS | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate + glutathione | - |
Homo sapiens | (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + glutathione | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | crystallization | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
Hematopoietic prostaglandin D synthase | i.e. H-PGDS | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
glutathione | - |
Homo sapiens |