Cloned (Comment) | Organism |
---|---|
expression of GST-tagged bb' fragment, residues P135-S357, and of b' fragment, residues L236-S357, in Escherichia coli strain Bl21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | loss of the two cysteines in the C-terminal a' domain increases the Km for substarte RNase A, and loss of an additional cysteine in the a domain resulted in an even further increase of the Km | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition of PDI oxido-reductase activity with di(o-aminobenzyl)-labeled oxidized glutathione by DELTA-somatostatin | Homo sapiens | |
Somatostatin | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
RNAse A | wild-type PDI | Homo sapiens | |
0.036 | - |
RNAse A | mutant PDI without Cys in domain a' | Homo sapiens | |
0.05 | - |
RNAse A | mutant PDI without two Cys in domains a' and a | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | PDI catalyzes disulfide bond formation in the endoplasmic reticulum | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P07237 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged bb' fragment and b' fragment from Escherichia coli strain Bl21(DE3) by glutathione affinity chromatography and gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bronchial epithelial cell | - |
Homo sapiens | - |
lung | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | PDI catalyzes disulfide bond formation in the endoplasmic reticulum | Homo sapiens | ? | - |
? | |
RNase A | PDI catalyzes the refolding of denatured bovine RNase A. The protein disulfide isomerase exhibits a saturable, substrate binding site. NMR structural analysis of peptide binding pocket of b and b' domains, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the protein disulfide isomerase exhibits a saturable, substrate binding site. NMR structural analysis of peptide binding pocket of b and b' domains, and interaction analysis of b and b' domains of PDI, the b' domain tends to form dimers, while the b domain moderates the tendency of the b' domain to dimerize and significantly slows interconversion, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
PDI | - |
Homo sapiens |
protein disulfide isomerase | - |
Homo sapiens |