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Literature summary for 5.3.4.1 extracted from
- Protein disulphide isomerase is required for signal peptide peptidase-mediated protein degradation (2010), EMBO J., 29, 363-375.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of myc-tagged mutant PDI in HeLa cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F258W/I272A | the mutant shows stronger binding of signal peptide peptidase, SPP, than the wild-type enzyme. PDI F258W/I272A-myc competes with endogenous PDI for SPP and remains attached to SPP, leading to a reduced pool of SPP available for US2-mediated degradation of MHC class I | Homo sapiens |
| additional information | knockdown of PDI by RNA-mediated interference inhibits the degradation of MHC class I molecules catalysed by human cytomegalovirus glycoprotein US2 but not by its functional homolog US11. Overexpression of the substrate-binding mutant of PDI, but not the catalytically inactive mutant, dominant-negatively inhibits US2-mediated dislocation of MHC class I molecules by preventing their release from US2. PDI associated with signal peptide peptidase, SPP, independently of US2 and knockdown of PDI inhibits SPP-mediated degradation of CD3d but not Derlin-1-dependent degradation of CFTR DeltaF508 | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | PDI specifically associates with signal peptide peptidase, SPP, independently of human cytomegalovirus glycoprotein US2, but not with Derlin-1 | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | transiently transfected with human wild-type US2, wild-type US11, or different cysteine mutants of US2 and US11 | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | PDI specifically associates with signal peptide peptidase, SPP, independently of human cytomegalovirus glycoprotein US2, but not with Derlin-1 | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PDI | - |
Homo sapiens |
| Protein disulphide isomerase | - |
Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the substrate binding, but not catalytic, activity of PDI is essential for the degradation of MHC class I HC by human cytomegalovirus glycoprotein US2, but not by US11, since oxidative folding of US2 is required for US2 function in inducing degradation of MHC class I molecules, PDI catalyses the release of MHC class I molecules from US2, function for PDI in SPP-mediated ERAD pathway, overview | Homo sapiens |
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