Cloned (Comment) | Organism |
---|---|
the gene encoding ERp27 is located at 14,958,24114,982,750 bp on chromosome 12 and has 7 exons, DNA and amino acid sequence determination and anaylsis, sequence comparison, expression of GFP-fusion ERp27 in COS-7 cell endoplasmic reticulum, expression of N-terminally His-tagged wild-type and mutant ERp57, and of PDI a, b, and b' domains, in Escherichia coli strain BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E231A/W232A/D233G | site-directed mutagenesis, the ERp27 mutant shows a similar structure as wild-type ERp57, but highly reduced binding to ERp57 compared to the wild-type enzyme | Homo sapiens |
E231K | site-directed mutagenesis, the ERp27 mutant shows a similar structure as wild-type ERp57, but highly reduced binding to ERp57 compared to the wild-type enzyme | Homo sapiens |
I196W | site-directed mutagenesis, the ERp27 mutant shows a structure and ERp57 binding similar to the wild-type enzyme | Homo sapiens |
additional information | construction of deletion mutants of ERp27 with deletions at the N terminus to P34 and/or at the C terminus to Leu257, overview | Homo sapiens |
R280A | site-directed mutagenesis, the mutant shows a structure reduced binding of ERp27, wild-type and mutants, as compared to the wild-type ERp57 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | ERp27 contains a putative secretory pathway signal sequence and a putative endoplasmic reticulum-retention signal, overview | Homo sapiens | 5783 | - |
additional information | subcellular localization study with wild-type and mutant enzyme, overview | Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27700 | - |
x * 27700, ERp27, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q96DN0 | ERp27 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant proteins from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bone marrow | expression of ERp27 | Homo sapiens | - |
kidney | expression of ERp27 | Homo sapiens | - |
lung | expression of ERp27 | Homo sapiens | - |
pancreas | high expression of ERp27 | Homo sapiens | - |
spleen | expression of ERp27 | Homo sapiens | - |
thymus | expression of ERp27 | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | ERp27 is non-catalytic and interacts with ERp57, ERp57 binds ERp27 by the Asp-Glu-Trp-Asp sequence in domain 2, reduced binding to R280A mutant ERp57, overview, it binds DELTA-somatostatin, the standard test peptide for protein disulfide isomerase-substrate binding, at its second domain, a significant conformational change in the b'-like domain of ERp27 occurs upon substrate binding, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27700, ERp27, SDS-PAGE | Homo sapiens |
More | ERp27 is a two-domain protein homologous to the non-catalytic b and b' domains of protein disulfide isomerase, domain structure, thioredoxin site structure and involved residues, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ERp27 | - |
Homo sapiens |
More | the enzyme belongs to the family of protein disulfide isomerases | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
glutathione | reduced and oxidized | Homo sapiens |