Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.4.1 extracted from

  • Alanen, H.I.; Williamson, R.A.; Howard, M.J.; Hatahet, F.S.; Salo, K.E.; Kauppila, A.; Kellokumpu, S.; Ruddock, L.W.
    ERp27, a new non-catalytic endoplasmic reticulum-located human protein disulfide isomerase family member, interacts with ERp57 (2006), J. Biol. Chem., 281, 33727-33738.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the gene encoding ERp27 is located at 14,958,241–14,982,750 bp on chromosome 12 and has 7 exons, DNA and amino acid sequence determination and anaylsis, sequence comparison, expression of GFP-fusion ERp27 in COS-7 cell endoplasmic reticulum, expression of N-terminally His-tagged wild-type and mutant ERp57, and of PDI a, b, and b' domains, in Escherichia coli strain BL21(DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
E231A/W232A/D233G site-directed mutagenesis, the ERp27 mutant shows a similar structure as wild-type ERp57, but highly reduced binding to ERp57 compared to the wild-type enzyme Homo sapiens
E231K site-directed mutagenesis, the ERp27 mutant shows a similar structure as wild-type ERp57, but highly reduced binding to ERp57 compared to the wild-type enzyme Homo sapiens
I196W site-directed mutagenesis, the ERp27 mutant shows a structure and ERp57 binding similar to the wild-type enzyme Homo sapiens
additional information construction of deletion mutants of ERp27 with deletions at the N terminus to P34 and/or at the C terminus to Leu257, overview Homo sapiens
R280A site-directed mutagenesis, the mutant shows a structure reduced binding of ERp27, wild-type and mutants, as compared to the wild-type ERp57 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum ERp27 contains a putative secretory pathway signal sequence and a putative endoplasmic reticulum-retention signal, overview Homo sapiens 5783
-
additional information subcellular localization study with wild-type and mutant enzyme, overview Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27700
-
x * 27700, ERp27, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q96DN0 ERp27
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant proteins from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
bone marrow expression of ERp27 Homo sapiens
-
kidney expression of ERp27 Homo sapiens
-
lung expression of ERp27 Homo sapiens
-
pancreas high expression of ERp27 Homo sapiens
-
spleen expression of ERp27 Homo sapiens
-
thymus expression of ERp27 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information ERp27 is non-catalytic and interacts with ERp57, ERp57 binds ERp27 by the Asp-Glu-Trp-Asp sequence in domain 2, reduced binding to R280A mutant ERp57, overview, it binds DELTA-somatostatin, the standard test peptide for protein disulfide isomerase-substrate binding, at its second domain, a significant conformational change in the b'-like domain of ERp27 occurs upon substrate binding, overview Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
? x * 27700, ERp27, SDS-PAGE Homo sapiens
More ERp27 is a two-domain protein homologous to the non-catalytic b and b' domains of protein disulfide isomerase, domain structure, thioredoxin site structure and involved residues, overview Homo sapiens

Synonyms

Synonyms Comment Organism
ERp27
-
Homo sapiens
More the enzyme belongs to the family of protein disulfide isomerases Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
glutathione reduced and oxidized Homo sapiens