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Literature summary for 5.3.4.1 extracted from

  • Tsai, C.W.; Duggan, P.F.; Shimp, R.L., Jr.; Miller, L.H.; Narum, D.L.
    Overproduction of Pichia pastoris or Plasmodium falciparum protein disulfide isomerase affects expression, folding and O-linked glycosylation of a malaria vaccine candidate expressed in P. pastoris (2005), J. Biotechnol., 121, 458-470.
    View publication on PubMed
Search for more literature for 5.3.4.1

Application

Application Comment Organism
biotechnology overexpression of Plasmodium falciparum PDI isozymes A and B for production of a disulfide-rich transmission-blocking vaccine candidate Pfs25 in Pichia pastoris, the expression level is enhance by co-expression of the endogenous Pichia pastoris enzyme in Pfs25 3fold, production method evaluation Komagataella pastoris
biotechnology overexpression of Plasmodium falciparum PDI isozymes A and B for production of a disulfide-rich transmission-blocking vaccine candidate Pfs25 in Pichia pastoris, the expression level is enhanced by co-expression of the endogenous Pichia pastoris enzyme in Pfs25 clone 3fold, production method evaluation Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment) Organism
gene pdi, DNA and amino avid sequence determination and analysis, expression of PDI in Pichia pastoris strain overexpressing the Plasmodium falciparum disulfide-rich, transmission-blocking vaccine candidate protein Pfs25, isozymes A and B, the co-expression results in 3fold enhanced levels of the Pfs25 isozymes A and B as well as in enhaced PpPDI expression, overview Komagataella pastoris
gene pdi, DNA and amino avid sequence determination and anaylsis, expression of PDI in Pichia pastoris strain overexpressing the Plasmodium falciparum disulfide-rich, transmission-blocking vaccine candidate protein Pfs25, isozymes A and B, the co-expression results in enhanced levels of the Pfs25 isozymes A and B as well as enhanced PfPDI expression, overview Plasmodium falciparum
overexpression of endogenous PDI in clone Pfs25 Komagataella pastoris
overexpression of wild-type isozymes A and B and of the chimeric PDI mutant in Pichia pastoris using the PFs25 clone, co-expression of Pichia pastoris PDI in Pfs25 Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
additional information replacement of the signal sequence and ER retention sequence by those of Pichia pastoris for elimination of glycosylation sites facilitating the recombinant expression in yeast Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Komagataella pastoris Q9C1Z8
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-
Plasmodium falciparum
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-
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Plasmodium falciparum AY919669 chimeric PDI mutant containing Pichia pastoris signal sequence and ER retention sequence
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Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein O-glycosylation Plasmodium falciparum

Synonyms

Synonyms Comment Organism
PDI
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 Plasmodium falciparum
PDI
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 Komagataella pastoris
protein disulfide isomerase
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 Plasmodium falciparum
protein disulfide isomerase
-
 Komagataella pastoris