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Literature summary for 5.3.4.1 extracted from
- Thermophilic fungal protein disulfide isomerase (1998), Methods Enzymol., 290, 50-59.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Bacillus brevis | Humicola insolens |
General Stability
| General Stability | Organism |
|---|---|
| stable to 0.2 M guanidine hydrochloride | Humicola insolens |
| unstable to 0.2 M guanidine hydrochloride | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
scrambled ribonuclease | scrambled | Bos taurus | |
| 0.0033 | - |
scrambled ribonuclease | scrambled | Humicola insolens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
2 * 57000, SDS-PAGE | Bos taurus |
| 60000 | - |
2 * 60000, SDS-PAGE | Humicola insolens |
| 120000 | - |
gel filtration | Humicola insolens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Humicola insolens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no glycoprotein | - |
Bos taurus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Humicola insolens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Humicola insolens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Insulin-(SS) + GSH | - |
Bos taurus | Insulin-(SH)2 + GSSG | - |
? |  |
| Insulin-(SS) + GSH | - |
Humicola insolens | Insulin-(SH)2 + GSSG | - |
? | |
| Proteins | refolding of scrambled ribonuclease | Humicola insolens | Proteins | with correct disulfide bonds | ? | |
| Proteins | native, reduced or with wrong disulfide bonds | Bos taurus | Proteins | with correct disulfide bonds | ? | |
| Proteins | native, reduced or with wrong disulfide bonds | Humicola insolens | Proteins | with correct disulfide bonds | ? | |
| Proteins | refolding of scrambled lysosyme | Humicola insolens | Proteins | with correct disulfide bonds | ? | |
| scrambled ribonuclease | - |
Bos taurus | ? | - |
? | |
| scrambled ribonuclease | - |
Humicola insolens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 60000, SDS-PAGE | Humicola insolens |
| dimer | 2 * 57000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Bos taurus |
| 40 | - |
- |
Humicola insolens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
t1/2: 27 days | Humicola insolens |
| 25 | - |
t1/2: 9 days | Bos taurus |
| 70 | - |
stable above | Humicola insolens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9 | - |
Bos taurus |
| 8 | 9 | - |
Humicola insolens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
stable | Humicola insolens |
| 5 | - |
unstable | Bos taurus |
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Release 2023.1 (January 2023)
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