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Literature summary for 5.3.3.9 extracted from

  • Polet, H.; Levine, L.
    Partial purification and characterization of prostaglandin A isomerase from rabbit serum (1975), Arch. Biochem. Biophys., 168, 96-103.
    View publication on PubMed

General Stability

General Stability Organism
8 M urea completely and irreversibly inactivates Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
NEM
-
Oryctolagus cuniculus
Urea 8 M, completely and irreversibly inactivates Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
prostaglandin A1 in presence of rabbit albumin Oryctolagus cuniculus
0.04
-
prostaglandin A1 in absence of rabbit albumin Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
gel filtration Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
serum
-
Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.133
-
-
Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid i.e. prostaglandin A1 Oryctolagus cuniculus (13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid
-
?
prostaglandin A1
-
Oryctolagus cuniculus ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
20 min, inactivation Oryctolagus cuniculus