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Literature summary for 5.3.3.9 extracted from

  • Ho, P.P.K.; Towner, R.D.; Sullivan, H.R.
    Purification of prostaglandin A isomerase from rabbit serum (1974), Prep. Biochem., 4, 257-271.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate) no effect at 1 mM Oryctolagus cuniculus
NEM 1 mM, 90% inhibition Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.054
-
prostaglandin A1
-
Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
-
Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
serum
-
Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.139
-
-
Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid i.e. prostaglandin A1 Oryctolagus cuniculus (13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid the product is prostaglandin C1, which is unstable under mild alkaline conditions and isomerizes to prostaglandin B1 ?
prostaglandin A1
-
Oryctolagus cuniculus ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Oryctolagus cuniculus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 75 about 30% of maximal activity at 30°C and 75°C Oryctolagus cuniculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
10 min, 50% loss of activity Oryctolagus cuniculus
60
-
10 min, rather stable at Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
Oryctolagus cuniculus