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Literature summary for 5.3.3.8 extracted from
- Human DELTA3,DELTA2-enoyl-CoA isomerase, type 2 a structural enzymology study on the catalytic role of its ACBP domain and helix-10 (2015), FEBS J., 282, 746-768 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting drop vapor diffusion method, using 100 mM MES pH 6.5, 1 M (NH4)2SO4 | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0834 | - |
3-trans-decenoyl-CoA | full length enzyme, at pH 8.5 and 25°C | Homo sapiens |  |
| 0.1385 | - |
3-trans-hexenoyl-CoA | full length enzyme, at pH 8.5 and 25°C | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Homo sapiens | 5739 | - |
| peroxisome | - |
Homo sapiens | 5777 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3Z)-enoyl CoA | Homo sapiens | - |
(2Z)-enoyl CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O75521 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Talon column chromatography and Superdex 200 gel filtration | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3Z)-enoyl CoA | - |
Homo sapiens | (2Z)-enoyl CoA | - |
? | |
| 3-trans-Decenoyl-CoA | - |
Homo sapiens | 2-trans-Decenoyl-CoA | - |
? | |
| 3-trans-Hexenoyl-CoA | - |
Homo sapiens | 2-trans-Hexenoyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 413000, calculated from amino acid sequence | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DELTA3,DELTA2-enoyl-CoA isomerase | - |
Homo sapiens |
| ECI2 | - |
Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 57 | - |
melting temperature | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 20.2 | - |
3-trans-hexenoyl-CoA | full length enzyme, at pH 8.5 and 25°C | Homo sapiens | |
| 56.3 | - |
3-trans-decenoyl-CoA | full length enzyme, at pH 8.5 and 25°C | Homo sapiens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 150 | - |
3-trans-hexenoyl-CoA | full length enzyme, at pH 8.5 and 25°C | Homo sapiens | |
| 670 | - |
3-trans-decenoyl-CoA | full length enzyme, at pH 8.5 and 25°C | Homo sapiens | |
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Release 2023.1 (January 2023)
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