Literature summary for 5.3.3.8 extracted from

Onwukwe, G.U.; Koski, M.K.; Pihko, P.; Schmitz, W.; Wierenga, R.K.
Structures of yeast peroxisomal DELTA3,DELTA2-enoyl-CoA isomerase complexed with acyl-CoA substrate analogues the importance of hydrogen-bond networks for the reactivity of the catalytic base and the oxyanion hole (2015), Acta Crystallogr. Sect. D, 71, 2178-2191 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme in the presence of 5 mM acetoacetyl-CoA or 1.8 mM octanoyl-CoA, sitting drop vapor diffusion method, using 100 mM MES pH 6.5, 1.6 M (NH4)2SO4, 10% (w/v) dioxane	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
DELTAH10	inactive	Saccharomyces cerevisiae
F268A	the mutant shows reduced activity compared to the wild type enzyme	Saccharomyces cerevisiae
N101A	the mutant shows reduced activity compared to the wild type enzyme	Saccharomyces cerevisiae
N101S	inactive	Saccharomyces cerevisiae
R100A	the mutant shows reduced activity compared to the wild type enzyme	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.05	-	3-trans-decenoyl-CoA	wild type enzyme, at pH 8.5 and 25°C	Saccharomyces cerevisiae
0.072	-	3-trans-hexenoyl-CoA	wild type enzyme, at pH 8.5 and 25°C	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
peroxisome	-	Saccharomyces cerevisiae	5777	-

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	Q05871	-	-

Purification (Commentary)

Purification (Comment)	Organism
Talon column chromatography and Superdex 200 gel filtration	Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.26	-	mutant enzyme F268A, with 3-trans-hexenoyl-CoA as substrate, at pH 8.5 and 25°C	Saccharomyces cerevisiae
0.35	-	mutant enzyme F268A, with 3-trans-decenoyl-CoA as substrate, at pH 8.5 and 25°C	Saccharomyces cerevisiae
0.4	-	mutant enzyme R100A, with 3-trans-decenoyl-CoA as substrate, at pH 8.5 and 25°C	Saccharomyces cerevisiae
0.5	-	mutant enzyme N101A, with 3-trans-hexenoyl-CoA as substrate, at pH 8.5 and 25°C	Saccharomyces cerevisiae
0.62	-	mutant enzyme N101A, with 3-trans-decenoyl-CoA as substrate, at pH 8.5 and 25°C	Saccharomyces cerevisiae
0.93	-	mutant enzyme R100A, with 3-trans-hexenoyl-CoA as substrate, at pH 8.5 and 25°C	Saccharomyces cerevisiae
1.93	-	wild type enzyme, with 3-trans-hexenoyl-CoA as substrate, at pH 8.5 and 25°C	Saccharomyces cerevisiae
4.4	-	wild type enzyme, with 3-trans-decenoyl-CoA as substrate, at pH 8.5 and 25°C	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-trans-Decenoyl-CoA	-	Saccharomyces cerevisiae	2-trans-Decenoyl-CoA	-	?
3-trans-Hexenoyl-CoA	-	Saccharomyces cerevisiae	2-trans-Hexenoyl-CoA	-	?

Synonyms

Synonyms	Comment	Organism
DELTA3,DELTA2-enoyl-CoA isomerase	-	Saccharomyces cerevisiae
ECI1	-	Saccharomyces cerevisiae
ECI2	-	Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
67	-	melting temperature	Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.7	-	3-trans-hexenoyl-CoA	wild type enzyme, at pH 8.5 and 25°C	Saccharomyces cerevisiae
4.4	-	3-trans-decenoyl-CoA	wild type enzyme, at pH 8.5 and 25°C	Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
20000	-	3-trans-hexenoyl-CoA	wild type enzyme, at pH 8.5 and 25°C	Saccharomyces cerevisiae
90000	-	3-trans-decenoyl-CoA	wild type enzyme, at pH 8.5 and 25°C	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)