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Literature summary for 5.3.3.8 extracted from

  • Goepfert, S.; Vidoudez, C.; Tellgren-Roth, C.; Delessert, S.; Hiltunen, J.K.; Poirier, Y.
    Peroxisomal DELTA(3),DELTA(2)-enoyl CoA isomerases and evolution of cytosolic paralogues in embryophytes (2008), Plant J., 56, 728-742.
    View publication on PubMed
Search for more literature for 5.3.3.8

Cloned(Commentary)

Cloned (Comment) Organism
heterologously expressed in Sacchcaromyces cerevisiae. In vivo enzyme activity tests of the proteins encoded by AtECI1, AtECI2, and AtECI3 by complementation of the Saccharomyces cerevisiae double mutant eciDELTAdci1DELTA with deletions of the genes, DELTA2,DELTA3-enoyl-CoA isomerase and DELTA3,4,DELTA2,4-dienoyl CoA isomerase Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information in the absence of DELTA3,DELTA2-enoyl CoA isomerase, complete beta-oxidation of (10Z)-heptadecanoic acid is blocked. In vivo enzyme activity tests of the proteins encoded by AtECI1, AtECI2, and AtECI3 by complementation of the Saccharomyces cerevisiae double mutant eciDELTAdci1DELTA with deletions of the genes, DELTA2,DELTA3-enoyl-CoA isomerase and DELTA3,4,DELTA2,4-dienoyl CoA isomerase Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol determined by producing constructs between enhanced yellow fluorescent protein (EYFP) at the N-terminus and the AtECI proteins at the C-terminus. The localization of EYFP-AtECI1 and EYFP-AtECI2 indicate that AtECI1 and AtECI2 are peroxisomal proteins. The EYFP-AtECI3 construct results in diffuse fluorescence throughout the cytosol and nucleus, indicating the absence of peroxisomal targeting. AtECI1, AtECI2, and AtECI3 show high overall homology to peroximal mammalian isomerases, but have only one coserved glutamate reisdue in an position similar to that in the mitochondrial isoemrases Arabidopsis thaliana 5829
-
nucleus determined by producing constructs between enhanced yellow fluorescent protein (EYFP) at the N-terminus and the AtECI proteins at the C-terminus. The localization of EYFP-AtECI1 and EYFP-AtECI2 indicate that AtECI1 and AtECI2 are peroxisomal proteins. The EYFP-AtECI3 construct results in diffuse fluorescence throughout the cytosol and nucleus, indicating the absence of peroxisomal targeting. AtECI1, AtECI2, and AtECI3 show high overall homology to peroximal mammalian isomerases, but have only one conserved glutamate reisdue in an position similar to that in the mitochondrial isoemrases Arabidopsis thaliana 5634
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peroxisome determined by producing constructs between enhanced yellow fluorescent protein (EYFP) at the N-terminus and the AtECI proteins at the C-terminus. The localization of EYFP-AtECI1 and EYFP-AtECI2 indicate that AtECI1 and AtECI2 are peroxisomal proteins. The EYFP-AtECI3 construct results in diffuse fluorescence throughout the cytosol and nucleus, indicating the absence of peroxisomal targeting. AtECI1, AtECI2, and AtECI3 show high overall homology to peroximal mammalian isomerases, but have only one coserved glutamate reisdue in an position similar to that in the mitochondrial isoemrases Arabidopsis thaliana 5777
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 AtECI1, AtECI2, AtECI3, all three proteins consist of 240 amino acids, predicted mass. AtECI2 and AtECI3 share 77% identity over the whole protein, and both are located in tandem on chromosome 4, separated by 1314 bp Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana enzyme participates in the degradation of unsaturated fatty acids through beta-oxidation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 genes AtECI1, AtECI2, AtECI3
-

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Arabidopsis thaliana
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3E)-undecenoyl CoA intermediate of beta-oxidation of (10Z)-heptadecenoic acid. In vivo enzyme activity tests of the proteins encoded by AtECI1, AtECI2, and AtECI3 by complementation of the Saccharomyces cerevisiae double mutant eciDELTAdci1DELTA with deletions of the genes, DELTA2,DELTA3-enoyl-CoA isomerase and DELTA3,4,DELTA2,4-dienoyl CoA isomerase Arabidopsis thaliana (2E)-undecenoyl CoA
-
 ?
(3E,7Z)-tridecedienoyl CoA intermediate of beta-oxidation of 10Z-heptadecenoic acid. In vivo enzyme activity tests of the proteins encoded by AtECI1, AtECI2, and AtECI3 by complementation of the Saccharomyces cerevisiae double mutant eciDELTAdci1DELTA with deletions of the genes, DELTA2,DELTA3-enoyl-CoA isomerase and DELTA3,4,DELTA2,4-dienoyl CoA isomerase Arabidopsis thaliana (2E,7Z)-tridecedienoyl CoA
-
 ?
(3Z)-nonenoyl CoA intermediate of beta-oxidation of (10Z)-heptadecenoic acid. In vivo enzyme activity tests of the proteins encoded by AtECI1, AtECI2, and AtECI3 by complementation of the Saccharomyces cerevisiae double mutant eciDELTAdci1DELTA with deletions of the genes, DELTA2,DELTA3-enoyl-CoA isomerase and DELTA3,4,DELTA2,4-dienoyl CoA isomerase Arabidopsis thaliana (2Z)-nonenoyl CoA
-
 ?
additional information enzyme participates in the degradation of unsaturated fatty acids through beta-oxidation Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
AtECI1
-
 Arabidopsis thaliana
AtECI2
-
 Arabidopsis thaliana
AtECI3
-
 Arabidopsis thaliana
DELTA3,DELTA2-enoyl CoA isomerase
-
 Arabidopsis thaliana
ECI
-
 Arabidopsis thaliana

pI Value

Organism Comment pI Value Maximum pI Value
Arabidopsis thaliana AtECI1
-
 6.7
Arabidopsis thaliana AtECI3
-
 7.3
Arabidopsis thaliana AtECI2
-
 8.6