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Literature summary for 5.3.3.7 extracted from

  • Yuhara, K.; Yonehara, H.; Hattori, T.; Kobayashi, K.; Kirimura, K.
    Enzymatic characterization and gene identification of aconitate isomerase, an enzyme involved in assimilation of trans-aconitic acid, from Pseudomonas sp. WU-0701 (2015), FEBS J., 282, 4257-4267 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
1,10-phenanthroline 177% activity at 1 mM Pseudomonas sp. WU-0701
dithiothreitol 166% activity at 1 mM Pseudomonas sp. WU-0701

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 9% residual activity at 1 mM Pseudomonas sp. WU-0701
EDTA 84% residual activity at 1 mM Pseudomonas sp. WU-0701
glycerol 78% residual activity at 20% (v/v) Pseudomonas sp. WU-0701
Hg2+ 5% residual activity at 1 mM Pseudomonas sp. WU-0701
Mo4+ 58% residual activity at 1 mM Pseudomonas sp. WU-0701

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.9
-
cis-aconitate at pH 6.0 and 37°C Pseudomonas sp. WU-0701
8
-
trans-aconitate at pH 6.0 and 37°C Pseudomonas sp. WU-0701

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 169% activity at 1 mM Pseudomonas sp. WU-0701
Cu2+ 147% activity at 1 mM Pseudomonas sp. WU-0701
Fe2+ 177% activity at 1 mM Pseudomonas sp. WU-0701
Mg2+ 183% activity at 1 mM Pseudomonas sp. WU-0701
Mn2+ 160% activity at 1 mM Pseudomonas sp. WU-0701
Ni2+ 157% activity at 1 mM Pseudomonas sp. WU-0701
Zn2+ 128% activity at 1 mM Pseudomonas sp. WU-0701

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25300
-
gel filtration Pseudomonas sp. WU-0701

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cis-aconitate Pseudomonas sp. WU-0701
-
trans-aconitate
-
r
trans-Aconitate Pseudomonas sp. WU-0701
-
cis-Aconitate
-
r

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. WU-0701 A0A0A1H8I4
-
-

Purification (Commentary)

Purification (Comment) Organism
CM-Sepharose column chromatography, Bio-Scale CHT5-I column chromatography, and Superdex 200 gel filtration Pseudomonas sp. WU-0701

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.16
-
cell-free extract, at pH 7.0 and 37°C Pseudomonas sp. WU-0701
1267
-
after 305fold purification, at pH 7.0 and 37°C Pseudomonas sp. WU-0701

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-aconitate
-
Pseudomonas sp. WU-0701 trans-aconitate
-
r
additional information no activity with citric acid, succinic acid, fumaric acid, and 2-oxoglutaric acid, and other dicarboxylic acids, such as itaconic acid, formic acid, citraconic acid, and maleic acid Pseudomonas sp. WU-0701 ?
-
?
trans-Aconitate
-
Pseudomonas sp. WU-0701 cis-Aconitate
-
r

Subunits

Subunits Comment Organism
monomer 1 * 25000, SDS-PAGE Pseudomonas sp. WU-0701

Synonyms

Synonyms Comment Organism
Aconitate isomerase
-
Pseudomonas sp. WU-0701
ais
-
Pseudomonas sp. WU-0701

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Pseudomonas sp. WU-0701

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 about 50% activity at 20°C, about 90-100% activity at 30-37°C, about 70% activity at 40°C, about 35% activity at 50°C, about 20% activity at 60 and 70°C Pseudomonas sp. WU-0701

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4500
-
cis-aconitate at pH 6.0 and 37°C Pseudomonas sp. WU-0701
15000
-
trans-aconitate at pH 6.0 and 37°C Pseudomonas sp. WU-0701

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Pseudomonas sp. WU-0701

pH Range

pH Minimum pH Maximum Comment Organism
5
-
more than 50% activity between pH 4.0 and 6.0 Pseudomonas sp. WU-0701

Cofactor

Cofactor Comment Organism Structure
additional information the enzyme does not require any cofactors such as NADH and NADPH Pseudomonas sp. WU-0701

KCat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
190
-
trans-aconitate at pH 6.0 and 37°C Pseudomonas sp. WU-0701
760
-
cis-aconitate at pH 6.0 and 37°C Pseudomonas sp. WU-0701