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Literature summary for 5.3.3.7 extracted from
- Purification and kinetic properties of aconitate isomerase from Pseudomonas putida (1971), Biochemistry, 10, 2253-2259.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| glycerol | increases activity | Pseudomonas putida |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.18 | 7.7 | cis-aconitate | Km-value is pH-dependent and increases from 0.18 mM at pH 7.15 to 7.7 mM at pH 9.25 | Pseudomonas putida | |
| 1.05 | 1.9 | cis-aconitate | in presence of 20% glycerol, over a 4fold range in enzyme concentration, the Km-value is inversely dependent on the enzyme concentration | Pseudomonas putida | |
| 9 | 25 | cis-aconitate | in absence of glycerol, over a 4fold range in enzyme concentration, the Km-value is inversely dependent on the enzyme concentration | Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78000 | - |
gel filtration | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
| Pseudomonas putida A 3.12 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Pseudomonas putida |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:trans-aconitate-grown cell | - |
Pseudomonas putida | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 116 | - |
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trans-Aconitate | - |
Pseudomonas putida | cis-Aconitate | - |
? | |
| trans-Aconitate | - |
Pseudomonas putida A 3.12 | cis-Aconitate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Pseudomonas putida |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.3 | pH 7.5: about 30% of maximal activity, pH 9.3: about 80% loss of activity | Pseudomonas putida |
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