Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glycerol | increases activity | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | 7.7 | cis-aconitate | Km-value is pH-dependent and increases from 0.18 mM at pH 7.15 to 7.7 mM at pH 9.25 | Pseudomonas putida | |
1.05 | 1.9 | cis-aconitate | in presence of 20% glycerol, over a 4fold range in enzyme concentration, the Km-value is inversely dependent on the enzyme concentration | Pseudomonas putida | |
9 | 25 | cis-aconitate | in absence of glycerol, over a 4fold range in enzyme concentration, the Km-value is inversely dependent on the enzyme concentration | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
gel filtration | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida A 3.12 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Pseudomonas putida |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:trans-aconitate-grown cell | - |
Pseudomonas putida | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
116 | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans-Aconitate | - |
Pseudomonas putida | cis-Aconitate | - |
? | |
trans-Aconitate | - |
Pseudomonas putida A 3.12 | cis-Aconitate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.3 | pH 7.5: about 30% of maximal activity, pH 9.3: about 80% loss of activity | Pseudomonas putida |