Cloned (Comment) | Organism |
---|---|
into a pASG-IBA3 vector for expression in Escherichia coli Rosetta cells | Eubacterium barkeri |
Crystallization (Comment) | Organism |
---|---|
the crystal structure of 3-methylitaconate-DELTA-isomerase is solved at a resolution of 2.7 A | Eubacterium barkeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7 | - |
methylitaconate | (R,S)-3-methylitaconate | Eubacterium barkeri | |
60 | - |
Itaconate | - |
Eubacterium barkeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40270 | - |
theoretical, without Strep-tag | Eubacterium barkeri |
41500 | - |
theoretical, including the Strep-tag | Eubacterium barkeri |
45000 | - |
apparent molecular mass, determined by SDS-PAGE | Eubacterium barkeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylitaconate | Eubacterium barkeri | - |
2,3-dimethylmaleate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eubacterium barkeri | Q0QLE6 | - |
- |
Purification (Comment) | Organism |
---|---|
affinity purification on a streptavidin column | Eubacterium barkeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
itaconate | - |
Eubacterium barkeri | methylmaleate | - |
? | |
methylitaconate | - |
Eubacterium barkeri | 2,3-dimethylmaleate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 40270 Da | Eubacterium barkeri |
Synonyms | Comment | Organism |
---|---|---|
3-methylitaconate-DELTA-isomerase | - |
Eubacterium barkeri |
MII | - |
Eubacterium barkeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
activity assay | Eubacterium barkeri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
Itaconate | - |
Eubacterium barkeri | |
1720 | - |
methylitaconate | (R,S)-3-methylitaconate | Eubacterium barkeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
activity assay | Eubacterium barkeri |
General Information | Comment | Organism |
---|---|---|
metabolism | 3-methylitaconate-DELTA-isomerase participates in the nicotinate fermentation pathway | Eubacterium barkeri |