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Literature summary for 5.3.3.6 extracted from

  • Velarde, M.; Macieira, S.; Hilberg, M.; Bröker, G.; Tu, S.-M.; Golding, B.T.; Pierik, A.J.; Buckel, W.; Messerschmidt, A.
    Crystal structure and putative mechanism of 3-methylitaconate-DELTA-isomerase from Eubacterium barkeri (2009), J. Mol. Biol., 391, 609-620.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into a pASG-IBA3 vector for expression in Escherichia coli Rosetta cells Eubacterium barkeri

Crystallization (Commentary)

Crystallization (Comment) Organism
the crystal structure of 3-methylitaconate-DELTA-isomerase is solved at a resolution of 2.7 A Eubacterium barkeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7
-
methylitaconate (R,S)-3-methylitaconate Eubacterium barkeri
60
-
Itaconate
-
Eubacterium barkeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40270
-
theoretical, without Strep-tag Eubacterium barkeri
41500
-
theoretical, including the Strep-tag Eubacterium barkeri
45000
-
apparent molecular mass, determined by SDS-PAGE Eubacterium barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methylitaconate Eubacterium barkeri
-
2,3-dimethylmaleate
-
r

Organism

Organism UniProt Comment Textmining
Eubacterium barkeri Q0QLE6
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity purification on a streptavidin column Eubacterium barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
itaconate
-
Eubacterium barkeri methylmaleate
-
?
methylitaconate
-
Eubacterium barkeri 2,3-dimethylmaleate
-
r

Subunits

Subunits Comment Organism
homotetramer 4 * 40270 Da Eubacterium barkeri

Synonyms

Synonyms Comment Organism
3-methylitaconate-DELTA-isomerase
-
Eubacterium barkeri
MII
-
Eubacterium barkeri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
activity assay Eubacterium barkeri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
Itaconate
-
Eubacterium barkeri
1720
-
methylitaconate (R,S)-3-methylitaconate Eubacterium barkeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
activity assay Eubacterium barkeri

General Information

General Information Comment Organism
metabolism 3-methylitaconate-DELTA-isomerase participates in the nicotinate fermentation pathway Eubacterium barkeri