Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(4S,5S)-(+/-)-5-chloro-3-methylmuconolactone | isomerization of (4R,5R)-(+/-)-5-chloro-3-methylmuconolactone | Cupriavidus necator |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.66 | - |
(4R,5R/4S,5S)-(+/-)-5-chloro-3-methylmuconolactone | - |
Cupriavidus necator | |
6 | - |
(4S)-(+)-muconolactone | - |
Cupriavidus necator | |
10.3 | - |
(4R,5S)-(+)-5-Chloromuconolactone | - |
Cupriavidus necator |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
10100 | - |
10 * 10100, SDS-PAGE | Cupriavidus necator |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4S)-Muconolactone | Cupriavidus necator | - |
? | - |
? | |
(4S)-Muconolactone | Cupriavidus necator JMP 134-1 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
- |
- |
Cupriavidus necator | - |
basonym Alcaligenes eutrophus | - |
Cupriavidus necator JMP 134-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cupriavidus necator |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:benzoate-grown cell | - |
Cupriavidus necator | - |
Storage Stability | Organism |
---|---|
4°C, pH 7.5, 100 mM potassium sodium phosphate, 3 months stable | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4R,5R)-(+/-)-5-chloro-3-methylmuconolactone | - |
Cupriavidus necator | 2-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid | - |
? | |
(4R,5R)-(+/-)-5-chloro-3-methylmuconolactone | - |
Cupriavidus necator JMP 134-1 | 2-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid | - |
? | |
(4R,5R/4S,5S)-(+/-)-5-chloro-3-methylmuconolactone | - |
Cupriavidus necator | 2-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid | - |
? | |
(4R,5S)-(+)-5-chloromuconolactone | - |
Cupriavidus necator | (2S)-chloro(5-oxo-4,5-dihydrofuran-2-yl)acetic acid | - |
? | |
(4R,5S)-(+)-5-chloromuconolactone | - |
Cupriavidus necator JMP 134-1 | (2S)-chloro(5-oxo-4,5-dihydrofuran-2-yl)acetic acid | - |
? | |
(4R,5S)-(+/-)-5-chloro-3-methylmuconolactone | preferred substrate over the natural substrate (4S)-muconolactone | Cupriavidus necator | (2S)-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid | - |
? | |
(4R,5S)-(+/-)-5-chloro-3-methylmuconolactone | preferred substrate over the natural substrate (4S)-muconolactone | Cupriavidus necator JMP 134-1 | (2S)-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid | - |
? | |
(4S)-Muconolactone | - |
Cupriavidus necator | ? | - |
? | |
(4S)-Muconolactone | - |
Cupriavidus necator JMP 134-1 | ? | - |
? | |
muconolactone | (4S)-(+)-muconolactone | Cupriavidus necator | (5-oxo-4,5-dihydrofuran-2-yl)acetic acid | - |
? | |
muconolactone | (4S)-(+)-muconolactone | Cupriavidus necator JMP 134-1 | (5-oxo-4,5-dihydrofuran-2-yl)acetic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | 10 * 10100, SDS-PAGE | Cupriavidus necator |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
20 min, stable up to | Cupriavidus necator |
90 | - |
20 min, 25% loss of activity | Cupriavidus necator |
100 | - |
- |
Cupriavidus necator |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
42.5 | - |
(4R,5R/4S,5S)-(+/-)-5-chloro-3-methylmuconolactone | - |
Cupriavidus necator | |
683 | - |
(4S)-(+)-muconolactone | - |
Cupriavidus necator | |
5950 | - |
(4R,5S)-(+)-5-Chloromuconolactone | - |
Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
5R/4R,5S)-(+/-)-5-chloro-3-methylmuconolactone, more active in potassium sodium phosphate buffer than in Tris/HCl or borate buffer | Cupriavidus necator |