Literature summary for 5.3.3.2 extracted from

de Ruyck, J.; Janczak, M.W.; Neti, S.S.; Rothman, S.C.; Schubert, H.L.; Cornish, R.M.; Matagne, A.; Wouters, J.; Poulter, C.D.
Determination of kinetics and the crystal structure of a novel type 2 isopentenyl diphosphate dimethylallyl diphosphate isomerase from Streptococcus pneumoniae (2014), ChemBioChem, 15, 1452-1458 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli M15[pREP4] cells	Streptococcus pneumoniae

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using HEPES buffer (100 mM, pH 7.5 with 2 M (NH4)2SO4)	Streptococcus pneumoniae

Inhibitors

Inhibitors	Comment	Organism	Structure
FMN	-	Streptococcus pneumoniae
isopentenyl diphosphate	-	Streptococcus pneumoniae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.04	-	isopentenyl diphosphate	at pH 7.0 and 37°C	Streptococcus pneumoniae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
173600	-	gel filtration	Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
isopentenyl diphosphate	Streptococcus pneumoniae	-	dimethylallyl diphosphate	-	r
isopentenyl diphosphate	Streptococcus pneumoniae CGSP14	-	dimethylallyl diphosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pneumoniae	B2ILS5	-	-
Streptococcus pneumoniae CGSP14	B2ILS5	-	-

Purification (Commentary)

Purification (Comment)	Organism
His-Trap column chromatography	Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
isopentenyl diphosphate	-	Streptococcus pneumoniae	dimethylallyl diphosphate	-	r
isopentenyl diphosphate	-	Streptococcus pneumoniae CGSP14	dimethylallyl diphosphate	-	r

Subunits

Subunits	Comment	Organism
homotetramer	4 * 39868, calculated from amino acid sequence	Streptococcus pneumoniae
homotetramer	4 * 39874, purified His-tagged enzyme, electrospray ionization mass spectrometry	Streptococcus pneumoniae

Synonyms

Synonyms	Comment	Organism
Idi-2	-	Streptococcus pneumoniae
type 2 isopentenyl diphosphate: dimethylallyl diphosphate isomerase	-	Streptococcus pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.141	-	isopentenyl diphosphate	at pH 7.0 and 37°C	Streptococcus pneumoniae

Cofactor

Cofactor	Comment	Organism	Structure
FMNH2	-	Streptococcus pneumoniae

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0064	-	FMN	at pH 7.0 and 37°C	Streptococcus pneumoniae
0.292	-	isopentenyl diphosphate	at pH 7.0 and 37°C	Streptococcus pneumoniae

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Release 2023.1 (January 2023)