Literature summary for 5.3.3.2 extracted from

Neti, S.S.; Eckert, D.M.; Poulter, C.D.
Construction of functional monomeric type 2 isopentenyl diphosphate dimethylallyl diphosphate isomerase (2016), Biochemistry, 55, 4229-4238 .

Search for more literature for 5.3.3.2

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli M15pREP4 cells	Streptococcus pneumoniae

Protein Variants

Protein Variants	Comment	Organism
N37A	the mutant is a monomeric but active enzyme with by 20°C lowered melting temperature and reduced binding affinities of FMN (40fold) and a minimal effect on the kcat value compared to the wild type enzyme	Streptococcus pneumoniae

Inhibitors

Inhibitors	Comment	Organism	Structure
FMN	-	Streptococcus pneumoniae
isopentenyl diphosphate	-	Streptococcus pneumoniae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00595	-	isopentenyl diphosphate	wild type enzyme, at anaerobic conditions, at pH 7.0 and 37°C	Streptococcus pneumoniae
0.0408	-	isopentenyl diphosphate	wild type enzyme, at aerobic conditions, at pH 7.0 and 37°C	Streptococcus pneumoniae
0.572	-	isopentenyl diphosphate	mutant enzyme N37A, at aerobic conditions, at pH 7.0 and 37°C	Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
isopentenyl diphosphate	Streptococcus pneumoniae	-	dimethylallyl diphosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pneumoniae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose resin column chromatography	Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
isopentenyl diphosphate	-	Streptococcus pneumoniae	dimethylallyl diphosphate	-	r

Subunits

Subunits	Comment	Organism
homotetramer	-	Streptococcus pneumoniae

Synonyms

Synonyms	Comment	Organism
Idi-2	-	Streptococcus pneumoniae
type 2 isopentenyl diphosphate:dimethylallyl diphosphate isomerase	-	Streptococcus pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.875	-	isopentenyl diphosphate	wild type enzyme, at anaerobic conditions, at pH 7.0 and 37°C	Streptococcus pneumoniae
0.983	-	isopentenyl diphosphate	wild type enzyme, at aerobic conditions, at pH 7.0 and 37°C	Streptococcus pneumoniae
1.31	-	isopentenyl diphosphate	mutant enzyme N37A, at aerobic conditions, at pH 7.0 and 37°C	Streptococcus pneumoniae

Cofactor

Cofactor	Comment	Organism	Structure
FMN	-	Streptococcus pneumoniae

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0064	-	FMN	wild type enzyme, at anaerobic conditions, at pH 7.0 and 37°C	Streptococcus pneumoniae
0.292	-	isopentenyl diphosphate	wild type enzyme, at aerobic conditions, at pH 7.0 and 37°C	Streptococcus pneumoniae

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)