Crystallization (Comment) | Organism |
---|---|
of wild-type and mutants Y104A, Y104F | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
Y104A | 0.1% of wild-type activity. Crystallization data. The M2+ metal-binding site is absent in the structure, but Mg2+ is still present and bound to C67, E87, and four water molecules | Escherichia coli |
Y104F | 0.1% of wild-type activity. Crystallization data. General fold of enzyme is similar to wild-type | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0095 | - |
isopentenyl diphosphate | wild-type, pH 7.4, 37°C | Escherichia coli | |
0.0142 | - |
isopentenyl diphosphate | mutant Y104F, pH 7.4, 37°C | Escherichia coli | |
0.0225 | - |
isopentenyl diphosphate | mutant Y104A, pH 7.4, 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | Q46822 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isopentenyl diphosphate | - |
Escherichia coli | dimethylallyl diphosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
48 | - |
melting temperature of mutant Y104A | Escherichia coli |
55 | - |
melting temperature of mutant Y104F | Escherichia coli |
69 | - |
melting temperature of wild-type | Escherichia coli |