Inhibitors | Comment | Organism | Structure |
---|---|---|---|
O2 | the overall reaction is sensitive to O2 | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0048 | - |
isopentenyl diphosphate | aerobic, presence of NADPH, pH 7.0, 37°C | Staphylococcus aureus | |
0.012 | - |
isopentenyl diphosphate | aerobic, presence of dithionite, pH 7.0, 37°C | Staphylococcus aureus | |
0.0168 | - |
isopentenyl diphosphate | anaerobic, presence of NADPH, pH 7.0, 37°C | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
type II enzyme | - |
Staphylococcus aureus IDI-2 | - |
type II enzyme | - |
Renatured (Comment) | Organism |
---|---|
reconstitution of apo-enzyme with 5-deaza-FMN results in an inactive enzyme, whereas reconstitution with 1-deaza-FMN leads to full recovery of activity | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isopentenyl diphosphate | - |
Staphylococcus aureus | dimethylallyl diphosphate | - |
? | |
isopentenyl diphosphate | - |
Staphylococcus aureus IDI-2 | dimethylallyl diphosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
isopentenyl diphosphate | aerobic, presence of NADPH, pH 7.0, 37°C | Staphylococcus aureus | |
0.57 | - |
isopentenyl diphosphate | aerobic, presence of dithionite, pH 7.0, 37°C | Staphylococcus aureus | |
0.69 | - |
isopentenyl diphosphate | anaerobic, presence of NADPH, pH 7.0, 37°C | Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | FMN must be in reduced state to be catalytically active | Staphylococcus aureus | |
NADPH | needed only in catalytic amounts to activate the enzyme for multiple turnovers. Hydride transfer from NADPH to reduce FMN is pro-S stereospecific | Staphylococcus aureus |