Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N,N-dimethyl-2-amino-1-ethyl diphosphate | transition state analogue, competitive | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | substrate binds to the inactive oxidized and active reduced form of enzyme with similar affinities | Thermus thermophilus | |
0.0056 | - |
isopentenyl diphosphate | pH 7.0, 37°C | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Kd value is 0.13 mM at pH 7.0, 37°C | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
type II enzyme, recombinant protein | - |
Purification (Comment) | Organism |
---|---|
recombinant protein, enzyme purified under aerobic conditions is inactive until the flavin cofactor is reduced by NADPH or dithionite or photochemically | Thermus thermophilus |
Renatured (Comment) | Organism |
---|---|
enzyme purified under aerobic conditions is inactive until the flavin cofactor is reduced by NADPH or dithionite or photochemically | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isopentenyl diphosphate | - |
Thermus thermophilus | dimethylallyl diphosphate | - |
? | |
additional information | substrate binds to the inactive oxidized and active reduced form of enzyme with similar affinities. Substrate-dependent accumulation of the neutral flavin semiquinone during both the flavoenzyme reduction and reoxidation processes | Thermus thermophilus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17.9 | - |
isopentenyl diphosphate | pH 7.0, 37°C | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | reduced flavin is required. The neutral semiquinone state of the flavin is stabilized thermodynamically relative to free FMN in solution. Kd value is 0.0047 mM at pH 7.0, 37°C | Thermus thermophilus | |
NADPH | required for reductive activation of inactive oxidized enzyme. Kd value is 0.11 mM at pH 7.0, 37°C | Thermus thermophilus |