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Literature summary for 5.3.3.2 extracted from

  • Laupitz, R.; Hecht, S.; Amslinger, S.; Zepeck, F.; Kaiser, J.; Richter, G.; Schramek, N.; Steinbacher, S.; Huber, R.; Arigoni, D.; Bacher, A.; Eisenreich, W.; Rohdich, F.
    Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways (2004), Eur. J. Biochem., 271, 2658-2669.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 10mM, relative activity 100% Bacillus subtilis
Co2+ 10mM, relative activity under 0.005% Bacillus subtilis
Cu2+ 10mM, relative activity under 0.005% Bacillus subtilis
Mg2+ 10mM, relative activity 65% Bacillus subtilis
Mn2+ 10mM, relative activity 17% Bacillus subtilis
Ni2+ 10mM, relative activity under 0.005% Bacillus subtilis
Zn2+ 10mM, relative activity 0.2% Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38460
-
mass spectrometry Bacillus subtilis
38460
-
calculated Bacillus subtilis
39000
-
SDS-PAGE Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.001
-
pyruvate, C13-labeled at positions 2 and 3, aerobic, pH 8, 37°C Bacillus subtilis
0.08
-
dimethylallyl diphosphate, C13-labeled at positions 3, 4 and 5, aerobic, pH 8, 37°C Bacillus subtilis
0.19
-
dimethylallyl diphosphate, anaerobic, pH 8, 37°C Bacillus subtilis
0.23
-
dimethylallyl diphosphate, aerobic, pH 8, 37°C Bacillus subtilis
0.62
-
isopentenyl diphosphate, anaerobic, pH 8, 37°C Bacillus subtilis
0.63
-
isopentenyl diphosphate, aerobic, pH 8, 37°C Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isopentenyl diphosphate
-
Bacillus subtilis dimethylallyl diphosphate
-
r
pyruvate
-
Bacillus subtilis lactate
-
?

Synonyms

Synonyms Comment Organism
Idi-2
-
Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
FMN required under aerobic and anaerobic conditions Bacillus subtilis
NADH or NADPH required under aerobic, not under anaerobic conditions Bacillus subtilis
NADPH or NADH required under aerobic, not under anaerobic conditions Bacillus subtilis