Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10mM, relative activity 100% | Bacillus subtilis | |
Co2+ | 10mM, relative activity under 0.005% | Bacillus subtilis | |
Cu2+ | 10mM, relative activity under 0.005% | Bacillus subtilis | |
Mg2+ | 10mM, relative activity 65% | Bacillus subtilis | |
Mn2+ | 10mM, relative activity 17% | Bacillus subtilis | |
Ni2+ | 10mM, relative activity under 0.005% | Bacillus subtilis | |
Zn2+ | 10mM, relative activity 0.2% | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38460 | - |
mass spectrometry | Bacillus subtilis |
38460 | - |
calculated | Bacillus subtilis |
39000 | - |
SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.001 | - |
pyruvate, C13-labeled at positions 2 and 3, aerobic, pH 8, 37°C | Bacillus subtilis |
0.08 | - |
dimethylallyl diphosphate, C13-labeled at positions 3, 4 and 5, aerobic, pH 8, 37°C | Bacillus subtilis |
0.19 | - |
dimethylallyl diphosphate, anaerobic, pH 8, 37°C | Bacillus subtilis |
0.23 | - |
dimethylallyl diphosphate, aerobic, pH 8, 37°C | Bacillus subtilis |
0.62 | - |
isopentenyl diphosphate, anaerobic, pH 8, 37°C | Bacillus subtilis |
0.63 | - |
isopentenyl diphosphate, aerobic, pH 8, 37°C | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isopentenyl diphosphate | - |
Bacillus subtilis | dimethylallyl diphosphate | - |
r | |
pyruvate | - |
Bacillus subtilis | lactate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Idi-2 | - |
Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | required under aerobic and anaerobic conditions | Bacillus subtilis | |
NADH | or NADPH required under aerobic, not under anaerobic conditions | Bacillus subtilis | |
NADPH | or NADH required under aerobic, not under anaerobic conditions | Bacillus subtilis |