Cloned (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
expression in Escherichia coli | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
K107A | significant decrease in catalytic activity when compared with the native enzyme | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.423 | - |
3-[(4S)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate | pH 8.1, 37°C | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the protein is a bi-cupin, with two putative active sites each containing a bound metal ion | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis | P39639 | - |
- |
Bacillus subtilis 168 | P39639 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-[(4R)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate | - |
Bacillus subtilis | (3Z)-3-[(4R)-4-hydroxycyclohex-2-en-1-ylidene]-2-oxopropanoate | - |
? | |
3-[(4R)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate | - |
Bacillus subtilis 168 | (3Z)-3-[(4R)-4-hydroxycyclohex-2-en-1-ylidene]-2-oxopropanoate | - |
? | |
3-[(4S)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate | - |
Bacillus subtilis | (3E)-3-[(4S)-4-hydroxycyclohex-2-en-1-ylidene]-2-oxopropanoate | - |
? | |
3-[(4S)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate | - |
Bacillus subtilis 168 | (3E)-3-[(4S)-4-hydroxycyclohex-2-en-1-ylidene]-2-oxopropanoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BacB | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Bacillus subtilis |
37 | - |
assay at | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24.52 | - |
3-[(4S)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate | pH 8.1, 37°C | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.1 | - |
assay at | Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of the dipeptide antibiotic bacilysin | Bacillus subtilis |