Cloned (Comment) | Organism |
---|---|
cDNA, expression in Arabidopsis thaliana | Ptilota filicina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53600 | - |
2 * 61000, SDS-PAGE, 2 * 55900, sequence calculation including the signal peptide, 2 * 53600, sequence calculation without the signal peptide | Ptilota filicina |
55900 | - |
2 * 61000, SDS-PAGE, 2 * 55900, sequence calculation including the signal peptide, 2 * 53600, sequence calculation without the signal peptide | Ptilota filicina |
61000 | - |
2 * 61000, SDS-PAGE, 2 * 55900, sequence calculation including the signal peptide, 2 * 53600, sequence calculation without the signal peptide | Ptilota filicina |
125000 | - |
sedimentation equilibrium utracentrifugation | Ptilota filicina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Ptilota filicina | PFI may be part of a stress tolerance mechanism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ptilota filicina | Q8W257 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | native PFI is N-glycosylated | Ptilota filicina |
proteolytic modification | translation of a preprotein containing a signal peptide of 21 amino acids that is removed during maturation | Ptilota filicina |
Purification (Comment) | Organism |
---|---|
- |
Ptilota filicina |
Storage Stability | Organism |
---|---|
-20°C, purified PFI, months, stable | Ptilota filicina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5Z,8Z,11Z,14Z)-eicosatetraenoate | arachidonic acid, conversion to the conjugated triene | Ptilota filicina | ? | - |
? | |
(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate | the preferred substrates are highly unsaturated free fatty acids such as eicosapentaenoic acid | Ptilota filicina | (5Z,7E,9E,14Z,17Z)-eicosapentaenoate | - |
? | |
additional information | PFI may be part of a stress tolerance mechanism | Ptilota filicina | ? | - |
? | |
polyenoic fatty acid | PFI catalyzes the isomerization of a wide range of substrates containing three or more methylene interrupted olefins into a Z,E,E conjugated triene functionality | Ptilota filicina | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 61000, SDS-PAGE, 2 * 55900, sequence calculation including the signal peptide, 2 * 53600, sequence calculation without the signal peptide | Ptilota filicina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Ptilota filicina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Ptilota filicina |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | bound to PFI, cDNA contains a flavin-binding motif near the mature N-terminus | Ptilota filicina |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Ptilota filicina | sequence calculation including the signal peptide | - |
4.9 |
Ptilota filicina | sequence calculation without the signal peptide | - |
4.9 |