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Literature summary for 5.3.3.13 extracted from

  • Zheng, W.; Wise, M.L.; Wyrick, A.; Metz, J.G.; Yuan, L.; Gerwick, W.H.
    Polyenoic fatty acid isomerase from the marine alga Ptilota filicina: protein characterization and functional expression of the cloned cDNA (2002), Arch. Biochem. Biophys., 401, 11-20.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cDNA, expression in Arabidopsis thaliana Ptilota filicina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53600
-
2 * 61000, SDS-PAGE, 2 * 55900, sequence calculation including the signal peptide, 2 * 53600, sequence calculation without the signal peptide Ptilota filicina
55900
-
2 * 61000, SDS-PAGE, 2 * 55900, sequence calculation including the signal peptide, 2 * 53600, sequence calculation without the signal peptide Ptilota filicina
61000
-
2 * 61000, SDS-PAGE, 2 * 55900, sequence calculation including the signal peptide, 2 * 53600, sequence calculation without the signal peptide Ptilota filicina
125000
-
sedimentation equilibrium utracentrifugation Ptilota filicina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Ptilota filicina PFI may be part of a stress tolerance mechanism ?
-
?

Organism

Organism UniProt Comment Textmining
Ptilota filicina Q8W257
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein native PFI is N-glycosylated Ptilota filicina
proteolytic modification translation of a preprotein containing a signal peptide of 21 amino acids that is removed during maturation Ptilota filicina

Purification (Commentary)

Purification (Comment) Organism
-
Ptilota filicina

Storage Stability

Storage Stability Organism
-20°C, purified PFI, months, stable Ptilota filicina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5Z,8Z,11Z,14Z)-eicosatetraenoate arachidonic acid, conversion to the conjugated triene Ptilota filicina ?
-
?
(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate the preferred substrates are highly unsaturated free fatty acids such as eicosapentaenoic acid Ptilota filicina (5Z,7E,9E,14Z,17Z)-eicosapentaenoate
-
?
additional information PFI may be part of a stress tolerance mechanism Ptilota filicina ?
-
?
polyenoic fatty acid PFI catalyzes the isomerization of a wide range of substrates containing three or more methylene interrupted olefins into a Z,E,E conjugated triene functionality Ptilota filicina ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 61000, SDS-PAGE, 2 * 55900, sequence calculation including the signal peptide, 2 * 53600, sequence calculation without the signal peptide Ptilota filicina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at Ptilota filicina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Ptilota filicina

Cofactor

Cofactor Comment Organism Structure
flavin bound to PFI, cDNA contains a flavin-binding motif near the mature N-terminus Ptilota filicina

pI Value

Organism Comment pI Value Maximum pI Value
Ptilota filicina sequence calculation including the signal peptide
-
4.9
Ptilota filicina sequence calculation without the signal peptide
-
4.9