Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.3.13 extracted from

  • Wise, M.L.; Rossi, J.; Gerwick, W.H.
    Binding site characterization of polyenoic fatty-acid isomerase from the marine alga Ptilota filicina (1997), Biochemistry, 36, 2985-2992.
    View publication on PubMed
Search for more literature for 5.3.3.13

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information study of the effect of the pH on kinetic parameters with several substrates Ptilota filicina
0.0019
-
 (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexenoate pH 7, 22°C Ptilota filicina
0.0096
-
 (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate pH 7, 22°C Ptilota filicina
0.0152
-
 (5Z,8Z,11Z,14Z)-eicosatetraenoyl-N-ethanolamide pH 9, 22°C Ptilota filicina
0.017
-
 (7Z,10Z,13Z,16Z)-docosatetraenoate pH 7, 22°C Ptilota filicina
0.0175
-
 (5Z,8Z,11Z,14Z)-eicosatetraenoyl-N-ethanolamide pH 7, 22°C Ptilota filicina
0.0255
-
 (5Z,8Z,11Z,14Z)-eicosatetraenoate pH 7, 22°C Ptilota filicina
0.0528
-
 (5Z,8Z,11Z)-eicosatrienoate pH 7, 22°C Ptilota filicina
0.15
-
 (6Z,9Z,12Z)-octadecatrienoate
-
 Ptilota filicina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 x * 61000, SDS-PAGE Ptilota filicina

Organism

Organism UniProt Comment Textmining
Ptilota filicina
-
-
-
Ptilota filicina
-
 Oregon red marine alga
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ptilota filicina

Reaction

Reaction Comment Organism Reaction ID
(5Z,8Z,11Z,14Z,17Z)-icosapentaenoate = (5Z,7E,9E,14Z,17Z)-icosapentaenoate mechansim of hydrogen transfers in the isomerization reaction including a diene intermediate Ptilota filicina
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Ptilota filicina

Storage Stability

Storage Stability Organism
-20°C, purified PFI, months, stable Ptilota filicina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexenoate excellent substrate Ptilota filicina (4Z,7Z,9E,11E,16Z,19Z)-docosahexenoate
-
 ?
(5Z,8Z,11Z)-eicosatrienoate
-
 Ptilota filicina ?
-
 ?
(5Z,8Z,11Z,14Z)-eicosatetraenoate arachidonic acid Ptilota filicina (5Z,7E,9E,14Z)-eicosatetraenoate
-
 ?
(5Z,8Z,11Z,14Z)-eicosatetraenoyl-N-ethanolamide anandamide Ptilota filicina (5Z,7E,9E,14Z)-eicosatetraenoyl-N-ethanolamide
-
 ?
(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate
-
 Ptilota filicina (5Z,7E,9E,14Z,17Z)-eicosapentaenoate
-
 ?
(6Z,9Z,12Z)-octadecatrienoate gamma-linolenic acid Ptilota filicina (6Z,8E,10E)-octadecatrienoate
-
 ?
(7Z,10Z,13Z,16Z)-docosatetraenoate adrenic acid Ptilota filicina ?
-
 ?
(8Z,11Z,14Z)-eicosatrienoate dihomo-gamma-linolenic acid, formation of two products: a conjugated triene and a conjugated diene Ptilota filicina ?
-
 ?
(9Z,12Z,15Z)-octadecatrienoate alpha-linolenic acid Ptilota filicina ?
-
 ?
additional information substrate binding site, PFI preferentially orients the substrate in the catalytic site with respect to the methyl terminus and likely reacts, preferentially, with the protonated form of the substrate, not: linoleic acid Ptilota filicina ?
-
 ?

Subunits

Subunits Comment Organism
? x * 61000, SDS-PAGE Ptilota filicina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Ptilota filicina

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.6
-
 (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate assuming one catalytic site per enzyme molecule Ptilota filicina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 below Ptilota filicina

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 pH-dependence profile of PFI, catalytically active over a wide pH-range with (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate as substrate Ptilota filicina

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 PFI is relatively stable at these pH extremes Ptilota filicina