Literature summary for 5.3.3.12 extracted from

Michard, Q.; Commo, S.; Belaidi, J.P.; Alleaume, A.M.; Michelet, J.F.; Daronnat, E.; Eilstein, J.; Duche, D.; Marrot, L.; Bernard, B.A.
TRP-2 specifically decreases WM35 cell sensitivity to oxidative stress (2008), Free Radic. Biol. Med., 44, 1023-1031.

Search for more literature for 5.3.3.12

Cloned(Commentary)

Cloned (Comment)	Organism
overexpressed in WM35 melanoma cell line as model system, endogenously expressing low levels of TRP-2. TRP-2 specifically decreases WM35 cell sensitivity to oxidative stress. Overexpreesion of enzyme in HEK-293 cells has no influence on cell sensitivity to oxidative stress	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	WM35-wild-type and WM35-C2 cells, containing overexpressed TRP-2 enzyme are incubated with various H2O2 concentrations to determine the survival rate. TRP-2 reduces oxidative stress-induced toxicity of WM35-C2 cells compared to the wild-type cells, at 300 microM H2O2 30% decrease of cell sensitivity. TRP-2 protects genomic DNA from oxidative stress damage: in wild-type WM35 cells the damaging effect on DNA is visible at 50 microM H2O2. WM35 cells containing the overexpressed TRP-2 show DNA damage after treatment with 100 microM H2O2. The overexpression of TRP-2 in HEK-293 cells shows no improvement of the cell sensitivity to oxidative stress	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
dopachrome tautomerase	melanogenic enzyme	Homo sapiens
TRP-2	melanogenic enzyme	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)