Any feedback?
Literature summary for 5.3.3.1 extracted from
- Intensified process for the purification of an enzyme from inclusion bodies using integrated expanded bed adsorption and refolding (2006), Biotechnol. Prog., 22, 1187-1193.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of C-terminally His6-tagged enzyme as insoluble protein in inclusion bodies in Escherichia coli strain BLR(DE3) | Comamonas testosteroni |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-Androstene-3,17-dione | Comamonas testosteroni | - |
4-Androstene-3,17-dione | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Comamonas testosteroni | P00947 | i.e. Pseudomonas testosteroni | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BLR(DE3) to homogeneity by expanded bed adsorption column utilizing immobilized metal affinity chromatography with denaturant concentration in the feed stream from 8 to 0 M urea, chemical extraction from cells, method optimization and validation, overview | Comamonas testosteroni |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| repeated adsorptive refolding in an expanded bed with gradient change in the feed-stream composition from denaturing buffer containing 8 M urea and 50 mM potassium phosphate, pH 6.8 to the refolding buffer containing 50 mM potassium phosphate, pH 6.8, constant liquid feed rate, method evaluation, overview | Comamonas testosteroni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-Androstene-3,17-dione | - |
Comamonas testosteroni | 4-Androstene-3,17-dione | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DELTA5-3-ketosteroid isomerase | - |
Comamonas testosteroni |
| ketosteroid isomerase | - |
Comamonas testosteroni |
| KSI | - |
Comamonas testosteroni |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Comamonas testosteroni |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Comamonas testosteroni |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
