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Literature summary for 5.3.3.1 extracted from

  • Thomas, J.L.; Boswell, E.L.; Scaccia, L.A.; Pletnev, V.; Umland, T.C.
    Identification of key amino acids responsible for the substantially higher affinities of human type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3b-HSD1) for substrates, coenzymes, and inhibitors relative to human 3b-HSD2 (2005), J. Biol. Chem., 280, 21321-21328.
    View publication on PubMedView publication on EuropePMC
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Inhibitors

Inhibitors Comment Organism Structure
epostane
-
 Homo sapiens
trilostane
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0175
-
 dehydroepiandrosterone mutant R240Q, isoform 2, 27°C, pH 7.4 Homo sapiens
0.0279
-
 dehydroepiandrosterone wild-type, isoform 1, 27°C, pH 7.4 Homo sapiens
0.033
-
 dehydroepiandrosterone mutant Q105M, isoform 1, 27°C, pH 7.4 Homo sapiens
0.0623
-
 dehydroepiandrosterone mutant Q240R, isoform 1, 27°C, pH 7.4 Homo sapiens
0.0808
-
 dehydroepiandrosterone mutant Q105M, isoform 2, 27°C, pH 7.4 Homo sapiens
0.0884
-
 dehydroepiandrosterone wild-type, isoform 2, 27°C, pH 7.4 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 bifunctional 3beta-hydroxysteroid dehydrogenase/isomerase, isoforms type 1 and 2
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dehydroepiandrosterone
-
 Homo sapiens 3beta-hydroxy-4-androsten-17-one
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1990
-
 dehydroepiandrosterone mutant Q240R, isoform 1, 27°C, pH 7.4 Homo sapiens
2064
-
 dehydroepiandrosterone mutant R240Q, isoform 2, 27°C, pH 7.4 Homo sapiens
2088
-
 dehydroepiandrosterone mutant Q105M, isoform 1, 27°C, pH 7.4 Homo sapiens
3012
-
 dehydroepiandrosterone wild-type, isoform 1, 27°C, pH 7.4 Homo sapiens
4002
-
 dehydroepiandrosterone mutant Q105M, isoform 2, 27°C, pH 7.4 Homo sapiens
4890
-
 dehydroepiandrosterone wild-type, isoform 2, 27°C, pH 7.4 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADH isomerase activity of enzyme requires NADH, Km- and kcat-value of isoform 1 wild-type are 0.0046 mM and 45 per min, resp. Km- and kcat-value of isoform 2 wild-type are 0.0126 mM and 99.1 per min, resp Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00007
-
 epostane mutant Q240R, isoform 1, 27°C, pH 7.4 Homo sapiens
0.00007
-
 epostane wild-type, isoform 1, 27°C, pH 7.4 Homo sapiens
0.0001
-
 trilostane wild-type, isoform 1, 27°C, pH 7.4 Homo sapiens
0.00012
-
 trilostane mutant R240Q, isoform 2, 27°C, pH 7.4 Homo sapiens
0.00013
-
 trilostane mutant Q240R, isoform 1, 27°C, pH 7.4 Homo sapiens
0.00021
-
 epostane mutant R240Q, isoform 2, 27°C, pH 7.4 Homo sapiens
0.00098
-
 epostane wild-type, isoform 2, 27°C, pH 7.4 Homo sapiens
0.00164
-
 epostane mutant Q105M, isoform 2, 27°C, pH 7.4 Homo sapiens
0.00166
-
 trilostane wild-type, isoform 2, 27°C, pH 7.4 Homo sapiens
0.00184
-
 epostane mutant Q105M, isoform 1, 27°C, pH 7.4 Homo sapiens
0.00207
-
 trilostane mutant Q105M, isoform 2, 27°C, pH 7.4 Homo sapiens
0.00208
-
 trilostane mutant Q105M, isoform 1, 27°C, pH 7.4 Homo sapiens