Crystallization (Comment) | Organism |
---|---|
mutant W92A, in complex with d-equilenin, decrease in conformational stability results from destabilization of surface hydrophobic layer | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
L125A/V127A | mutation in small exterior hydrophobic cluster, decrease in conformational stability | Pseudomonas putida |
L125F/V127F | mutation in small exterior hydrophobic cluster, slight increase in stability | Pseudomonas putida |
W92A | mutation in small exterior hydrophobic cluster, decrease in conformational stability. Crystallization data | Pseudomonas putida |
W92A/L125A/V127A | mutation in small exterior hydrophobic cluster, decrease in conformational stability | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0499 | - |
5-androstene-3,17-dione | wild-type, 25°C, pH 7.0 | Pseudomonas putida | |
0.0795 | - |
5-androstene-3,17-dione | mutant L125F/V127F, 25°C, pH 7.0 | Pseudomonas putida | |
0.0995 | - |
5-androstene-3,17-dione | mutant W92A, 25°C, pH 7.0 | Pseudomonas putida | |
0.1185 | - |
5-androstene-3,17-dione | mutant W92A/L125A/V127A, 25°C, pH 7.0 | Pseudomonas putida | |
0.1375 | - |
5-androstene-3,17-dione | mutant L125A/V127A, 25°C, pH 7.0 | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
biotype B | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-Androstene-3,17-dione | - |
Pseudomonas putida | 4-Androstene-3,17-dione | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12000 | - |
5-androstene-3,17-dione | mutant W92A/L125A/V127A, 25°C, pH 7.0 | Pseudomonas putida | |
12700 | - |
5-androstene-3,17-dione | mutant W92A, 25°C, pH 7.0 | Pseudomonas putida | |
12900 | - |
5-androstene-3,17-dione | mutant L125A/V127A, 25°C, pH 7.0 | Pseudomonas putida | |
17600 | - |
5-androstene-3,17-dione | mutant L125F/V127F, 25°C, pH 7.0 | Pseudomonas putida | |
21200 | - |
5-androstene-3,17-dione | wild-type, 25°C, pH 7.0 | Pseudomonas putida |