Crystallization (Comment) | Organism |
---|---|
crystals of mutant enzyme F116W are grown by hanging-drop method | Comamonas testosteroni |
Protein Variants | Comment | Organism |
---|---|---|
F116W | the turnover-number for 5-androstene-3,17-dione is lowered 4.42fold, the KM-value is 3.1fold lower than the Km-value of the wild-type enzyme | Comamonas testosteroni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0486 | - |
5-androstene-3,17-dione | 25°C, pH 7.0, mutant enzyme F116W | Comamonas testosteroni | |
0.1528 | - |
5-androstene-3,17-dione | 25°C, pH 7.0, wild-type enzyme | Comamonas testosteroni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | P00947 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5(10)-estrene-3,17-dione | nonsticky substrate | Comamonas testosteroni | estr-4-en-3,17-dione | - |
? | |
5-Androstene-3,17-dione | - |
Comamonas testosteroni | 4-Androstene-3,17-dione | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TI-WT | - |
Comamonas testosteroni |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7155 | - |
5-androstene-3,17-dione | 25°C, pH 7.0, mutant enzyme F116W | Comamonas testosteroni | |
7160 | - |
5-androstene-3,17-dione | 25°C, pH 7.0, mutant enzyme F116W | Comamonas testosteroni | |
31680 | - |
5-androstene-3,17-dione | 25°C, pH 7.0, wild-type enzyme | Comamonas testosteroni | |
31700 | - |
5-androstene-3,17-dione | 25°C, pH 7.0, wild-type enzyme | Comamonas testosteroni |