Any feedback?
Literature summary for 5.3.3.1 extracted from
- Human placental 3beta-hydroxy-5-ene-steroid dehydrogenase and steroid 5-->4-ene-isomerase: purification from mitochondria and kinetic profiles, biophysical characterization of the purified mitochondrial and microsomal enzymes (1989), J. Steroid Biochem., 33, 209-217.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | mild | Homo sapiens |  |
| CdCl2 | moderate | Homo sapiens | |
| CuCl2 | strong | Homo sapiens | |
| FeCl2 | moderate | Homo sapiens | |
| ZnCl2 | moderate | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0093 | - |
5-Pregnene-3,20-dione | - |
Homo sapiens | |
| 0.0276 | - |
5-androstene-3,17-dione | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Homo sapiens | - |
- |
| mitochondrion | - |
Homo sapiens | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
2 * 41000, SDS-PAGE | Homo sapiens |
| 77000 | - |
enzyme complex of 3beta-hydroxy-5-ene-steroid dehydrogenase and steroid DELTA-isomerase gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
enzyme complex of 3beta-hydroxy-5-ene-steroid dehydrogenase and Steroid DELTA-isomerase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| enzyme complex of 3beta-hydroxy-5-ene-steroid dehydrogenase and steroid 5 to 4 isomerase | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| placenta | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 4.3% loss of activity after 2 weeks | Homo sapiens |
| -70°C, 6 months, no loss of activity | Homo sapiens |
| 22°C, 31.5% loss of activity after 4 days | Homo sapiens |
| 4°C, 12.5% loss of activity after 7 days | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-Androstene-3,17-dione | - |
Homo sapiens | 4-Androstene-3,17-dione | - |
? | |
| 5-Pregnene-3,20-dione | - |
Homo sapiens | 4-Pregnene-3,20-dione | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 41000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Homo sapiens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | 42 | 22°C: about 60% of maximal activity, 40°C: about 40% of maximal activity | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
31.5% loss of activity after 4 days | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | pH 6.5: about 60% of maximal activity, pH 8.0: about 40% of maximal activity, pH 6.3: no activity | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
