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show all sequences of 5.3.2.7

Crystal structure of bifunctional aldos-2-ulose dehydratase/isomerase from Phanerochaete chrysosporium with the reaction intermediate ascopyrone M

Claesson, M.; Lindqvist, Y.; Madrid, S.; Sandalova, T.; Fiskesund, R.; Yu, S.; Schneider, G.; J. Mol. Biol. 417, 279-293 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Hansenula polymorpha
Phanerochaete chrysosporium
Crystallization (Commentary)
Crystallization
Organism
hanging drop vapor diffusion method, using 0.1 M 4-morpholineethanesulfonic acid (pH 6.0), 0.2 M MgCl2 and 16% (w/v) polyethylene glycol 6000
Phanerochaete chrysosporium
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
contains zinc
Phanerochaete chrysosporium
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
98700
-
2 * 98700, SDS-PAGE
Phanerochaete chrysosporium
98746
-
2 * 98746, calculated from amino acid sequence
Phanerochaete chrysosporium
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ascopyrone M
Phanerochaete chrysosporium
-
microthecin
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Phanerochaete chrysosporium
P84193
cf. EC 4.2.1.110
-
Purification (Commentary)
Commentary
Organism
-
Phanerochaete chrysosporium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ascopyrone M
-
728150
Phanerochaete chrysosporium
microthecin
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 98700, SDS-PAGE; 2 * 98746, calculated from amino acid sequence
Phanerochaete chrysosporium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
for microthecin formation
Phanerochaete chrysosporium
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Phanerochaete chrysosporium
isoelectric focusing
5.3
5.2
Phanerochaete chrysosporium
calculated from amino acid sequence
-
5.5
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Hansenula polymorpha
Phanerochaete chrysosporium
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapor diffusion method, using 0.1 M 4-morpholineethanesulfonic acid (pH 6.0), 0.2 M MgCl2 and 16% (w/v) polyethylene glycol 6000
Phanerochaete chrysosporium
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
contains zinc
Phanerochaete chrysosporium
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
98700
-
2 * 98700, SDS-PAGE
Phanerochaete chrysosporium
98746
-
2 * 98746, calculated from amino acid sequence
Phanerochaete chrysosporium
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ascopyrone M
Phanerochaete chrysosporium
-
microthecin
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Phanerochaete chrysosporium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ascopyrone M
-
728150
Phanerochaete chrysosporium
microthecin
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 98700, SDS-PAGE; 2 * 98746, calculated from amino acid sequence
Phanerochaete chrysosporium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
for microthecin formation
Phanerochaete chrysosporium
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Phanerochaete chrysosporium
isoelectric focusing
5.3
5.2
Phanerochaete chrysosporium
calculated from amino acid sequence
-
5.5
Other publictions for EC 5.3.2.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728150
Claesson
Crystal structure of bifunctio ...
Phanerochaete chrysosporium
J. Mol. Biol.
417
279-293
2012
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667822
Yu
The anhydrofructose pathway an ...
Anthracobia melaloma
Biochim. Biophys. Acta
1760
1314-1322
2006
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650418
Yu
Enzymatic description of the a ...
Anthracobia melaloma
Biochim. Biophys. Acta
1672
120-129
2004
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