BRENDA - Enzyme Database show
show all sequences of 5.3.2.5

RuBisCO-like proteins as the enolase enzyme in the methionine salvage pathway: functional and evolutionary relationships between RuBisCO-like proteins and photosynthetic RuBisCO

Ashida, H.; Saito, Y.; Nakano, T.; Tandeau de Marsac, N.; Sekowska, A.; Danchin, A.; Yokota, A.; J. Exp. Bot. 59, 1543-1554 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
Bacillus subtilis RLPalpha-1 sequence determination and analysis, and phylogenetic analysis
Bacillus subtilis
Geobacillus kaustophilis RLPalpha-1 sequence determination and analysis, and phylogenetic analysis
Geobacillus kaustophilus
Microcystis aeruginosa RLPalpha-1 sequence determination and analysis, and phylogenetic analysis
Microcystis aeruginosa
Rhodopseudomonas palustris RLPalpha-1 sequence determination and analysis, and phylogenetic analysis
Rhodopseudomonas palustris
Rhodospirillum rubrum RLPalpha-1 sequence determination and analysis, and phylogenetic analysis
Rhodospirillum rubrum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Geobacillus kaustophilus
Mg2+
required
Rhodopseudomonas palustris
Mg2+
required
Rhodospirillum rubrum
Mg2+
required
Bacillus subtilis
Mg2+
required
Microcystis aeruginosa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-(methylthio)-2,3-dioxopentyl phosphate
Bacillus subtilis
-
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate
-
-
?
5-(methylthio)-2,3-dioxopentyl phosphate
Rhodopseudomonas palustris
-
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate
-
-
?
5-(methylthio)-2,3-dioxopentyl phosphate
Rhodospirillum rubrum
-
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate
-
-
?
5-(methylthio)-2,3-dioxopentyl phosphate
Microcystis aeruginosa
-
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate
-
-
?
5-(methylthio)-2,3-dioxopentyl phosphate
Geobacillus kaustophilus
-
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
-
-
-
Geobacillus kaustophilus
-
-
-
Microcystis aeruginosa
-
-
-
Rhodopseudomonas palustris
-
-
-
Rhodospirillum rubrum
-
-
-
Reaction
Reaction
Commentary
Organism
5-(methylsulfanyl)-2,3-dioxopentyl phosphate = 2-hydroxy-5-(methylsulfanyl)-3-oxopent-1-enyl phosphate
reaction mechanism, overview
Bacillus subtilis
5-(methylsulfanyl)-2,3-dioxopentyl phosphate = 2-hydroxy-5-(methylsulfanyl)-3-oxopent-1-enyl phosphate
reaction mechanism, overview
Geobacillus kaustophilus
5-(methylsulfanyl)-2,3-dioxopentyl phosphate = 2-hydroxy-5-(methylsulfanyl)-3-oxopent-1-enyl phosphate
reaction mechanism, overview
Microcystis aeruginosa
5-(methylsulfanyl)-2,3-dioxopentyl phosphate = 2-hydroxy-5-(methylsulfanyl)-3-oxopent-1-enyl phosphate
reaction mechanism, overview
Rhodopseudomonas palustris
5-(methylsulfanyl)-2,3-dioxopentyl phosphate = 2-hydroxy-5-(methylsulfanyl)-3-oxopent-1-enyl phosphate
reaction mechanism, overview
Rhodospirillum rubrum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-(methylthio)-2,3-dioxopentyl phosphate
-
693411
Bacillus subtilis
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate
-
-
-
?
5-(methylthio)-2,3-dioxopentyl phosphate
-
693411
Rhodopseudomonas palustris
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate
-
-
-
?
5-(methylthio)-2,3-dioxopentyl phosphate
-
693411
Rhodospirillum rubrum
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate
-
-
-
?
5-(methylthio)-2,3-dioxopentyl phosphate
-
693411
Microcystis aeruginosa
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate
-
-
-
?
5-(methylthio)-2,3-dioxopentyl phosphate
-
693411
Geobacillus kaustophilus
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
Bacillus subtilis RLPalpha-1 sequence determination and analysis, and phylogenetic analysis
Bacillus subtilis
Geobacillus kaustophilis RLPalpha-1 sequence determination and analysis, and phylogenetic analysis
Geobacillus kaustophilus
Microcystis aeruginosa RLPalpha-1 sequence determination and analysis, and phylogenetic analysis
Microcystis aeruginosa
Rhodopseudomonas palustris RLPalpha-1 sequence determination and analysis, and phylogenetic analysis
Rhodopseudomonas palustris
Rhodospirillum rubrum RLPalpha-1 sequence determination and analysis, and phylogenetic analysis
Rhodospirillum rubrum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Geobacillus kaustophilus
Mg2+
required
Rhodopseudomonas palustris
Mg2+
required
Rhodospirillum rubrum
Mg2+
required
Bacillus subtilis
Mg2+
required
Microcystis aeruginosa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-(methylthio)-2,3-dioxopentyl phosphate
Bacillus subtilis
-
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate
-
-
?
5-(methylthio)-2,3-dioxopentyl phosphate
Rhodopseudomonas palustris
-
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate
-
-
?
5-(methylthio)-2,3-dioxopentyl phosphate
Rhodospirillum rubrum
-
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate
-
-
?
5-(methylthio)-2,3-dioxopentyl phosphate
Microcystis aeruginosa
-
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate
-
-
?
5-(methylthio)-2,3-dioxopentyl phosphate
Geobacillus kaustophilus
-
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-(methylthio)-2,3-dioxopentyl phosphate
-
693411
Bacillus subtilis
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate
-
-
-
?
5-(methylthio)-2,3-dioxopentyl phosphate
-
693411
Rhodopseudomonas palustris
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate
-
-
-
?
5-(methylthio)-2,3-dioxopentyl phosphate
-
693411
Rhodospirillum rubrum
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate
-
-
-
?
5-(methylthio)-2,3-dioxopentyl phosphate
-
693411
Microcystis aeruginosa
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate
-
-
-
?
5-(methylthio)-2,3-dioxopentyl phosphate
-
693411
Geobacillus kaustophilus
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate
-
-
-
?
General Information
General Information
Commentary
Organism
evolution
the Bacillus subtilis enzyme belongs to the RLPalpha-1 enzyme group, functional and evolutionary relationships between Bacillus subtilis RLP of the methionine salvage pathway, other RLPs, and photosynthetic RuBisCO, overview
Bacillus subtilis
evolution
the Geobacillus kaustophilis enzyme belongs to the RLPalpha-1 enzyme group, functional and evolutionary relationships between Bacillus subtilis RLP of the methionine salvage pathway, other RLPs, and photosynthetic RuBisCO, overview
Geobacillus kaustophilus
evolution
the Microcystis aeruginosa enzyme belongs to the RLPalpha-1 enzyme group, functional and evolutionary relationships between Bacillus subtilis RLP of the methionine salvage pathway, other RLPs, and photosynthetic RuBisCO, overview
Microcystis aeruginosa
evolution
the Rhodopseudomonas palustris enzyme belongs to the RLPalpha-1 enzyme group, functional and evolutionary relationships between Bacillus subtilis RLP of the methionine salvage pathway, other RLPs, and photosynthetic RuBisCO, overview
Rhodopseudomonas palustris
evolution
the Rhodospirillum rubrum enzyme belongs to the RLPalpha-1 enzyme group, functional and evolutionary relationships between Bacillus subtilis RLP of the methionine salvage pathway, other RLPs, and photosynthetic RuBisCO, overview
Rhodospirillum rubrum
metabolism
Bacillus subtilis RLP is involved in the methionine salvage pathway, overview
Bacillus subtilis
metabolism
Geobacillus kaustophilis RLP is involved in the methionine salvage pathway, overview
Geobacillus kaustophilus
metabolism
Microcystis aeruginosa RLP is involved in the methionine salvage pathway, overview
Microcystis aeruginosa
metabolism
Rhodopseudomonas palustris RLP is involved in the methionine salvage pathway, it utilizes the methionine salvage pathway because it can grow using 5-methyladenosine as the sole source of sulfur, although the reaction step of DK-MTP-1-P enolase may be catalysed by a photosynthetic form II RuBisCO and not by RLPs, overview
Rhodopseudomonas palustris
metabolism
Rhodospirillum rubrum RLP is involved in the methionine salvage pathway, it utilizes the methionine salvage pathway because it can grow using 5-methyladenosine as the sole source of sulfur, although the reaction step of DK-MTP-1-P enolase may be catalysed by a photosynthetic form II RuBisCO and not by RLPs, overview
Rhodospirillum rubrum
additional information
conserved residues Lys123, Lys175, Lys201, Asp203, and Glu204 that are essential for the enolization reaction of DK-MTP-1-P enolase
Bacillus subtilis
additional information
conserved residues Lys123, Lys175, Lys201, Asp203, and Glu204 that are essential for the enolization reaction of DK-MTP-1-P enolase
Geobacillus kaustophilus
additional information
conserved residues Lys123, Lys175, Lys201, Asp203, and Glu204 that are essential for the enolization reaction of DK-MTP-1-P enolase
Microcystis aeruginosa
additional information
conserved residues Asn123, Lys175, Lys201, Asp203, and His204 are essential for the enolization reaction of DK-MTP-1-P enolase
Rhodopseudomonas palustris
additional information
conserved residues Asn123, Lys175, Lys201, Asp203, and His204 are essential for the enolization reaction of DK-MTP-1-P enolase
Rhodospirillum rubrum
physiological function
Bacillus subtilis RLP catalyses an analogous reaction to photosynthetic RuBisCO since its substrate resembles the structure of RuBP, rationalizing the tight linkage between RLPs and RuBisCO. But Bacillus subtilis RLP functions as an enzyme in a different metabolic pathway from that of RuBisCO, overview
Bacillus subtilis
General Information (protein specific)
General Information
Commentary
Organism
evolution
the Bacillus subtilis enzyme belongs to the RLPalpha-1 enzyme group, functional and evolutionary relationships between Bacillus subtilis RLP of the methionine salvage pathway, other RLPs, and photosynthetic RuBisCO, overview
Bacillus subtilis
evolution
the Geobacillus kaustophilis enzyme belongs to the RLPalpha-1 enzyme group, functional and evolutionary relationships between Bacillus subtilis RLP of the methionine salvage pathway, other RLPs, and photosynthetic RuBisCO, overview
Geobacillus kaustophilus
evolution
the Microcystis aeruginosa enzyme belongs to the RLPalpha-1 enzyme group, functional and evolutionary relationships between Bacillus subtilis RLP of the methionine salvage pathway, other RLPs, and photosynthetic RuBisCO, overview
Microcystis aeruginosa
evolution
the Rhodopseudomonas palustris enzyme belongs to the RLPalpha-1 enzyme group, functional and evolutionary relationships between Bacillus subtilis RLP of the methionine salvage pathway, other RLPs, and photosynthetic RuBisCO, overview
Rhodopseudomonas palustris
evolution
the Rhodospirillum rubrum enzyme belongs to the RLPalpha-1 enzyme group, functional and evolutionary relationships between Bacillus subtilis RLP of the methionine salvage pathway, other RLPs, and photosynthetic RuBisCO, overview
Rhodospirillum rubrum
metabolism
Bacillus subtilis RLP is involved in the methionine salvage pathway, overview
Bacillus subtilis
metabolism
Geobacillus kaustophilis RLP is involved in the methionine salvage pathway, overview
Geobacillus kaustophilus
metabolism
Microcystis aeruginosa RLP is involved in the methionine salvage pathway, overview
Microcystis aeruginosa
metabolism
Rhodopseudomonas palustris RLP is involved in the methionine salvage pathway, it utilizes the methionine salvage pathway because it can grow using 5-methyladenosine as the sole source of sulfur, although the reaction step of DK-MTP-1-P enolase may be catalysed by a photosynthetic form II RuBisCO and not by RLPs, overview
Rhodopseudomonas palustris
metabolism
Rhodospirillum rubrum RLP is involved in the methionine salvage pathway, it utilizes the methionine salvage pathway because it can grow using 5-methyladenosine as the sole source of sulfur, although the reaction step of DK-MTP-1-P enolase may be catalysed by a photosynthetic form II RuBisCO and not by RLPs, overview
Rhodospirillum rubrum
additional information
conserved residues Lys123, Lys175, Lys201, Asp203, and Glu204 that are essential for the enolization reaction of DK-MTP-1-P enolase
Bacillus subtilis
additional information
conserved residues Lys123, Lys175, Lys201, Asp203, and Glu204 that are essential for the enolization reaction of DK-MTP-1-P enolase
Geobacillus kaustophilus
additional information
conserved residues Lys123, Lys175, Lys201, Asp203, and Glu204 that are essential for the enolization reaction of DK-MTP-1-P enolase
Microcystis aeruginosa
additional information
conserved residues Asn123, Lys175, Lys201, Asp203, and His204 are essential for the enolization reaction of DK-MTP-1-P enolase
Rhodopseudomonas palustris
additional information
conserved residues Asn123, Lys175, Lys201, Asp203, and His204 are essential for the enolization reaction of DK-MTP-1-P enolase
Rhodospirillum rubrum
physiological function
Bacillus subtilis RLP catalyses an analogous reaction to photosynthetic RuBisCO since its substrate resembles the structure of RuBP, rationalizing the tight linkage between RLPs and RuBisCO. But Bacillus subtilis RLP functions as an enzyme in a different metabolic pathway from that of RuBisCO, overview
Bacillus subtilis
Other publictions for EC 5.3.2.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728615
Nakano
MtnBD is a multifunctional fus ...
Tetrahymena thermophila
PLoS ONE
8
e67385
2013
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727007
Warlick
Mechanistic diversity in the R ...
Geobacillus kaustophilus, no activity in Rhodospirillum rubrum
Biochemistry
51
9470-9479
2012
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714022
Nakano
An evolutionally conserved Lys ...
Bacillus subtilis
Biochem. Biophys. Res. Commun.
392
212-216
2010
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3
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2
715360
Singh
Roles of RubisCO and the Rubis ...
Rhodospirillum rubrum, Rhodospirillum rubrum Str-2
J. Bacteriol.
192
1324-1331
2010
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3
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701475
Tamura
Structure of the apo decarbamy ...
Bacillus subtilis
Acta Crystallogr. Sect. D
65
942-951
2009
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693411
Ashida
RuBisCO-like proteins as the e ...
Bacillus subtilis, Geobacillus kaustophilus, Microcystis aeruginosa, Rhodopseudomonas palustris, Rhodospirillum rubrum
J. Exp. Bot.
59
1543-1554
2008
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678268
Imker
Mechanistic diversity in the R ...
Bacillus subtilis 168, Bacillus subtilis, Geobacillus kaustophilus
Biochemistry
46
4077-4089
2007
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2
1
3
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2
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6
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717772
Carre-Mlouka
A new rubisco-like protein coe ...
Microcystis aeruginosa
J. Biol. Chem.
281
24462-24471
2006
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2
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718388
Ashida
Was photosynthetic RuBisCO rec ...
Bacillus subtilis
Res. Microbiol.
156
611-618
2005
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706755
Ashida
A functional link between RuBi ...
Bacillus subtilis
Science
302
286-290
2003
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3
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669181
Myers
Purification and characterizat ...
Klebsiella pneumoniae, Klebsiella pneumoniae CG253
J. Biol. Chem.
268
24785-24791
1993
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-
-
-
1
-
-
-
-
-
1
-
-
-
1
2
2
-
-
-
1
-
-
1
-
4
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-