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Literature summary for 5.3.2.5 extracted from

  • Myers, R.W.; Wray, J.W.; Fish, S.; Abeles, R.H.
    Purification and characterization of an enzyme involved in oxidative carbon-carbon bond cleavage reactions in the methionine salvage pathway of Klebsiella pneumoniae (1993), J. Biol. Chem., 268, 24785-24791.
    View publication on PubMed
Search for more literature for 5.3.2.5

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Klebsiella pneumoniae strain CG253 leading to 5-6fold increased activity compared to the parent strain Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by phosphate Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25500
-
 gel filtration Klebsiella pneumoniae
28300
-
 1 * 28300, SDS-PAGE Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,3-diketo-1-phosphohexane Klebsiella pneumoniae an alternate substrate for the methionine salvage pathway 2-hydroxy-3-keto-l-phospho-l-hexene
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-
Klebsiella pneumoniae CG253
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by fractionation with help of protamine suzlfate, ammonium sulfate, and P-60, followed by anion exchange and hydrophobic interaction chromatography Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 overall pathway activity, purified enzyme, pH 7.5, 30°C Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-diketo-1-phosphohexane an alternate substrate for the methionine salvage pathway Klebsiella pneumoniae 2-hydroxy-3-keto-l-phospho-l-hexene
-
 ?
2,3-diketo-1-phosphohexane an analogue to 2,3-diketo-5-methylthio-1-phosphopentane Klebsiella pneumoniae 2-hydroxy-3-keto-l-phospho-l-hexene a chromophoric intermediate compound, product determination by NMR analysis ?
2,3-diketo-l-phospho-5-thiomethylpentane
-
 Klebsiella pneumoniae ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 28300, SDS-PAGE Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Klebsiella pneumoniae

General Information

General Information Comment Organism
metabolism Klebsiella pneumoniae converts 2,3-diketo-5-methylthio-l-phosphopentane to formate, 2-keto-4-methylthiobutyrate, and 3-methylthiopropionate. 2,3-diketo-1-phosphohexane, the desthio analogue of the naturally occurring diketone, can serve as alternate substrate in the methionine salvage pathway Klebsiella pneumoniae