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Literature summary for 5.3.2.2 extracted from
- Isolation and properties of oxaloacetate keto-enol tautomerases from bovine heart mitochondria (1988), Biochim. Biophys. Acta, 936, 10-19.
General Stability
| General Stability | Organism |
|---|---|
| oxaloacetate tautomerase-1 resists freezing and thawing when dissolved in potassium phosphate buffer, pH 7.8 | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | enzyme form OAT-2 is inhibited, enzyme form OAT-1 not | Bos taurus |  |
| Maleate | - |
Bos taurus | |
| malonate | enzyme form OAT-2 is inhibited, enzyme form OAT-1 not | Bos taurus | |
| oxalate | - |
Bos taurus | |
| Oxaloacetic acid diethylester | enzyme form OAT-1 is inhibited, enzyme form OAT-2 not | Bos taurus | |
| phenylpyruvate | enzyme form OAT-2 is inhibited, enzyme form OAT-1 not | Bos taurus | |
| phosphoenolpyruvate | enzyme form OAT-2 is inhibited, enzyme form OAT-1 not | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.045 | - |
enol-oxaloacetate | enzyme form OAT-1 | Bos taurus | |
| 0.068 | - |
keto-oxaloacetate | enzyme form OAT-1 | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrial matrix | about 30% of the tautomerase activity in the matrix are represented by oxaloacetate tautomerase-1 and about 70% by oxaloacetate tautomerase-2 | Bos taurus | 5759 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
enzyme form OAT-1, gel filtration | Bos taurus |
| 80000 | - |
enzyme form OAT-2, gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| enzyme form OAT-1 and enzyme form OAT-2 | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Bos taurus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, oxaloacetate tautomerase-1 is quite stable for several days | Bos taurus |
| 0°C, oxaloacetate tautomerase-2 gradually loses activity within several days | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| keto-Oxaloacetate | r | Bos taurus | Enol-oxaloacetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 37000, enzyme form OAT-1, SDS-PAGE | Bos taurus |
| monomer | 1 * 80000, enzyme form OAT-2, SDS-PAGE | Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 26.7 | - |
enol-oxaloacetate | enzyme form OAT-2, 25°C, pH 9.0 | Bos taurus | |
| 45.7 | - |
enol-oxaloacetate | enzyme form OAT-1, 25°C, pH 9.0 | Bos taurus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 10 | oxaloacetate tautomerase-1 | Bos taurus |
| 9 | - |
oxaloacetate tautomerase-2 | Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | pH 7.0: about 50% of maximal activity, pH 8.5-10.0: maximal activity, oxaloacetate tautomerase-1 | Bos taurus |
| 7.7 | 9.5 | pH 7.7: about 20% of maximal activity, pH 9.5: about 40% of maximal activity, oxaloacetate tautomerase-2 | Bos taurus |
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