BRENDA - Enzyme Database
show all sequences of 5.3.2.2

Oxalacetate keto-enol tautomerase. Purification and characterization

Annett, R.G.; Kosicki, G.W.; J. Biol. Chem. 244, 2059-2067 (1969)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-oxoglutarate
-
Sus scrofa
acetoacetate
-
Sus scrofa
citrate
-
Sus scrofa
CuCl2
-
Sus scrofa
Diethyloxaloacetate
-
Sus scrofa
DL-malic acid
-
Sus scrofa
HgCl2
-
Sus scrofa
KCl
-
Sus scrofa
Lactate
-
Sus scrofa
LiCl
-
Sus scrofa
malonate
-
Sus scrofa
MgCl2
-
Sus scrofa
NaCl
-
Sus scrofa
PCMB
-
Sus scrofa
pyruvate
-
Sus scrofa
Urea
-
Sus scrofa
ZnCl2
-
Sus scrofa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sus scrofa
-
-
-
Purification (Commentary)
Commentary
Organism
-
Sus scrofa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.06
-
-
Sus scrofa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
keto-Oxaloacetate
-
2874
Sus scrofa
Enol-oxaloacetate
-
2874
Sus scrofa
-
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
-
40% loss of activity after 10 min
Sus scrofa
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
3.5
-
quick denaturation below
Sus scrofa
4
-
at pH 8 or pH 4 more stable than at values in between
Sus scrofa
8
-
at pH 8 or pH 4 more stable than at values in between
Sus scrofa
8.5
-
quick denaturation above
Sus scrofa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-oxoglutarate
-
Sus scrofa
acetoacetate
-
Sus scrofa
citrate
-
Sus scrofa
CuCl2
-
Sus scrofa
Diethyloxaloacetate
-
Sus scrofa
DL-malic acid
-
Sus scrofa
HgCl2
-
Sus scrofa
KCl
-
Sus scrofa
Lactate
-
Sus scrofa
LiCl
-
Sus scrofa
malonate
-
Sus scrofa
MgCl2
-
Sus scrofa
NaCl
-
Sus scrofa
PCMB
-
Sus scrofa
pyruvate
-
Sus scrofa
Urea
-
Sus scrofa
ZnCl2
-
Sus scrofa
Purification (Commentary) (protein specific)
Commentary
Organism
-
Sus scrofa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.06
-
-
Sus scrofa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
keto-Oxaloacetate
-
2874
Sus scrofa
Enol-oxaloacetate
-
2874
Sus scrofa
-
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
-
40% loss of activity after 10 min
Sus scrofa
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
3.5
-
quick denaturation below
Sus scrofa
4
-
at pH 8 or pH 4 more stable than at values in between
Sus scrofa
8
-
at pH 8 or pH 4 more stable than at values in between
Sus scrofa
8.5
-
quick denaturation above
Sus scrofa
Other publictions for EC 5.3.2.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
2877
Vinogradov
Regulation of succinate dehydr ...
Bos taurus
Adv. Enzyme Regul.
28
271-280
1989
1
-
-
-
-
-
5
3
1
1
2
1
-
1
-
-
-
-
-
1
-
-
3
1
-
-
1
3
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
5
-
3
1
1
2
1
-
-
-
-
-
1
-
-
3
1
-
-
1
3
-
-
-
-
-
-
-
-
-
-
2878
Belikova
Identification of the high-mol ...
Bos taurus
FEBS Lett.
246
17-20
1989
-
-
-
-
-
-
4
1
2
1
-
-
-
2
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
1
2
1
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2879
Belikova
Isolation and properties of ox ...
Bos taurus
Biochim. Biophys. Acta
936
10-19
1988
-
-
-
-
-
1
7
2
1
-
2
-
-
2
-
-
1
-
-
1
-
2
1
1
-
-
-
2
2
2
-
-
-
-
-
-
-
-
-
-
-
1
-
7
-
2
1
-
2
-
-
-
-
1
-
1
-
2
1
1
-
-
-
2
2
2
-
-
-
-
-
-
-
-
2880
Belikova
Oxidation of malate by mitocho ...
Bos taurus
Biochim. Biophys. Acta
936
1-9
1988
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2876
Johnson
Stereochemistry and function o ...
Sus scrofa
J. Biol. Chem.
261
4535-4541
1986
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2875
Wesenberg
Localization of oxalacetate ke ...
Rattus norvegicus, Sus scrofa
Can. J. Biochem.
54
233-237
1976
-
-
-
-
-
-
-
-
6
-
-
-
-
2
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2874
Annett
Oxalacetate keto-enol tautomer ...
Sus scrofa
J. Biol. Chem.
244
2059-2067
1969
-
-
-
-
-
-
17
-
-
-
-
-
-
1
-
-
1
-
-
1
1
-
1
-
-
-
1
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
17
-
-
-
-
-
-
-
-
-
1
-
1
1
-
1
-
-
-
1
-
-
-
4
-
-
-
-
-
-
-