Literature summary for 5.3.1.B3 extracted from

Yoshida, H.; Teraoka, M.; Yoshihara, A.; Izumori, K.; Kamitori, S.
Overexpression, crystallization and preliminary X-ray diffraction analysis of L-ribose isomerase from Acinetobacter sp. strain DL-28 (2011), Acta Crystallogr. Sect. F, 67, 1281-1284.

Search for more literature for 5.3.1.B3

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli JM109 cells	Acinetobacter sp.

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 0.2 M NaCl, 30% (w/v) PEG 400, 0.1 M HEPES pH 7.5 or 18-23% (w/v) PEG 400, 0.1 M HEPES pH 7.5, 0.16-0.2 M NaCl with or without 10-20 mM hexamminecobalt(III) chloride	Acinetobacter sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28700	-	x * 28700, N-terminally His-tagged enzyme, SDS-PAGE	Acinetobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-ribulose	Acinetobacter sp.	-	L-ribose	-	r
L-ribulose	Acinetobacter sp. DL-28	-	L-ribose	-	r

Organism

Organism	UniProt	Comment	Textmining
Acinetobacter sp.	Q93UQ5	-	-
Acinetobacter sp. DL-28	Q93UQ5	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap affinity column chromatography, and gel filtration	Acinetobacter sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-ribulose	-	Acinetobacter sp.	L-ribose	-	r
L-ribulose	-	Acinetobacter sp. DL-28	L-ribose	-	r

Subunits

Subunits	Comment	Organism
?	x * 28700, N-terminally His-tagged enzyme, SDS-PAGE	Acinetobacter sp.

Synonyms

Synonyms	Comment	Organism
L-RI	-	Acinetobacter sp.

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Release 2023.1 (January 2023)