Application | Comment | Organism |
---|---|---|
drug development | PGI might be a good target for species-specific drug design | Trypanosoma brucei brucei |
Cloned (Comment) | Organism |
---|---|
expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Trypanosoma brucei brucei |
Crystallization (Comment) | Organism |
---|---|
enzyme complexed with glucose 6-phosphate, X-ray diffraction structure determination and analysis at 1.6 A resolution | Rattus norvegicus |
purified recombinant His-tagged enzyme complexed with glucose 6-phosphate, by hanging drop vapor diffusion method at room temperature, 0.004 ml of protein solution with 28.4 mg/ml protein and 5 mM fructose 6-phosphate is mixed with 0.002 ml of reservoir solution containing 10% PEG 3350, 50 mM sodium citrate, and 50 mM dithiothreitol, a few days, X-ray diffraction structure determination and analysis at 1.6 A resolution. Although fructose 6-phosphate is added to the crystallization mixture, the enzyme shows bound gluose 6-phosphate at its active site in the crystals | Trypanosoma brucei brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-phosphoarabinonhydroxamic acid | - |
Rattus norvegicus | |
5-phosphoarabinonhydroxamic acid | competitive inhibition | Trypanosoma brucei brucei | |
Agaricic acid | irreversible inhibition | Trypanosoma brucei brucei | |
additional information | no inhibition by suramin, an anti-trypanosomal drug, and agaricic acid | Rattus norvegicus | |
suramin | an anti-trypanosomal drug | Trypanosoma brucei brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate | Rattus norvegicus | - |
D-fructose 6-phosphate | - |
r | |
D-glucose 6-phosphate | Trypanosoma brucei brucei | - |
D-fructose 6-phosphate | - |
r | |
D-glucose 6-phosphate | Trypanosoma brucei brucei Treu 927 | - |
D-fructose 6-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Trypanosoma brucei brucei | P13377 | GUTat 10.1 | - |
Trypanosoma brucei brucei Treu 927 | P13377 | GUTat 10.1 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by immobilized metal ion affinity chromatography | Trypanosoma brucei brucei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
48.5 | - |
purified recombinant His-tagged enzyme | Trypanosoma brucei brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate | - |
Rattus norvegicus | D-fructose 6-phosphate | - |
r | |
D-glucose 6-phosphate | - |
Trypanosoma brucei brucei | D-fructose 6-phosphate | - |
r | |
D-glucose 6-phosphate | the glucose 6-phosphate molecule is bound in an extended conformation in the active site of PGI, substrate binding structure, overview | Trypanosoma brucei brucei | D-fructose 6-phosphate | - |
r | |
D-glucose 6-phosphate | - |
Trypanosoma brucei brucei Treu 927 | D-fructose 6-phosphate | - |
r | |
D-glucose 6-phosphate | the glucose 6-phosphate molecule is bound in an extended conformation in the active site of PGI, substrate binding structure, overview | Trypanosoma brucei brucei Treu 927 | D-fructose 6-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | structure and active site conformation, overview | Trypanosoma brucei brucei |
Synonyms | Comment | Organism |
---|---|---|
Phosphoglucose isomerase | - |
Rattus norvegicus |
Phosphoglucose isomerase | - |
Trypanosoma brucei brucei |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
suramin | - |
Trypanosoma brucei brucei |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00005 | - |
- |
Trypanosoma brucei brucei | 5-phosphoarabinonhydroxamic acid | |
0.0002 | - |
- |
Rattus norvegicus | 5-phosphoarabinonhydroxamic acid | |
0.01 | - |
- |
Trypanosoma brucei brucei | Agaricic acid |
General Information | Comment | Organism |
---|---|---|
metabolism | PGI is the second enzyme of glycolysis | Rattus norvegicus |
metabolism | PGI is the second enzyme of glycolysis | Trypanosoma brucei brucei |