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Literature summary for 5.3.1.9 extracted from

  • Arsenieva, D.; Appavu, B.; Mazock, G.; Jeffery, C.
    Crystal structure of phosphoglucose isomerase from Trypanosoma brucei complexed with glucose-6-phosphate at 1.6 A resolution (2009), Proteins, 74, 72-80.
    View publication on PubMed

Application

Application Comment Organism
drug development PGI might be a good target for species-specific drug design Trypanosoma brucei brucei

Cloned(Commentary)

Cloned (Comment) Organism
expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) Trypanosoma brucei brucei

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme complexed with glucose 6-phosphate, X-ray diffraction structure determination and analysis at 1.6 A resolution Rattus norvegicus
purified recombinant His-tagged enzyme complexed with glucose 6-phosphate, by hanging drop vapor diffusion method at room temperature, 0.004 ml of protein solution with 28.4 mg/ml protein and 5 mM fructose 6-phosphate is mixed with 0.002 ml of reservoir solution containing 10% PEG 3350, 50 mM sodium citrate, and 50 mM dithiothreitol, a few days, X-ray diffraction structure determination and analysis at 1.6 A resolution. Although fructose 6-phosphate is added to the crystallization mixture, the enzyme shows bound gluose 6-phosphate at its active site in the crystals Trypanosoma brucei brucei

Inhibitors

Inhibitors Comment Organism Structure
5-phosphoarabinonhydroxamic acid
-
Rattus norvegicus
5-phosphoarabinonhydroxamic acid competitive inhibition Trypanosoma brucei brucei
Agaricic acid irreversible inhibition Trypanosoma brucei brucei
additional information no inhibition by suramin, an anti-trypanosomal drug, and agaricic acid Rattus norvegicus
suramin an anti-trypanosomal drug Trypanosoma brucei brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate Rattus norvegicus
-
D-fructose 6-phosphate
-
r
D-glucose 6-phosphate Trypanosoma brucei brucei
-
D-fructose 6-phosphate
-
r
D-glucose 6-phosphate Trypanosoma brucei brucei Treu 927
-
D-fructose 6-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-
Trypanosoma brucei brucei P13377 GUTat 10.1
-
Trypanosoma brucei brucei Treu 927 P13377 GUTat 10.1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by immobilized metal ion affinity chromatography Trypanosoma brucei brucei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
48.5
-
purified recombinant His-tagged enzyme Trypanosoma brucei brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate
-
Rattus norvegicus D-fructose 6-phosphate
-
r
D-glucose 6-phosphate
-
Trypanosoma brucei brucei D-fructose 6-phosphate
-
r
D-glucose 6-phosphate the glucose 6-phosphate molecule is bound in an extended conformation in the active site of PGI, substrate binding structure, overview Trypanosoma brucei brucei D-fructose 6-phosphate
-
r
D-glucose 6-phosphate
-
Trypanosoma brucei brucei Treu 927 D-fructose 6-phosphate
-
r
D-glucose 6-phosphate the glucose 6-phosphate molecule is bound in an extended conformation in the active site of PGI, substrate binding structure, overview Trypanosoma brucei brucei Treu 927 D-fructose 6-phosphate
-
r

Subunits

Subunits Comment Organism
dimer structure and active site conformation, overview Trypanosoma brucei brucei

Synonyms

Synonyms Comment Organism
Phosphoglucose isomerase
-
Rattus norvegicus
Phosphoglucose isomerase
-
Trypanosoma brucei brucei

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.29
-
suramin
-
Trypanosoma brucei brucei

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00005
-
-
Trypanosoma brucei brucei 5-phosphoarabinonhydroxamic acid
0.0002
-
-
Rattus norvegicus 5-phosphoarabinonhydroxamic acid
0.01
-
-
Trypanosoma brucei brucei Agaricic acid

General Information

General Information Comment Organism
metabolism PGI is the second enzyme of glycolysis Rattus norvegicus
metabolism PGI is the second enzyme of glycolysis Trypanosoma brucei brucei