Cloned (Comment) | Organism |
---|---|
overexpression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) | Plasmodium falciparum |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, mixing 0.002 ml of both protein solution and reservoir solution, the latter containing 38% v/v PEG 400 and 0.2 M calcium acetate in 0.1 M sodium cacodylate-HCl, pH 6.5, equilibration over 0.5 ml of reservoir solution, 1 week, X-ray diffraction structure determination and analysis at 1.5 A resolution | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
x * 65000, recombinant enzyme, SDS-PAGE | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate | Plasmodium falciparum | - |
D-fructose 6-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration to homogeneity | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate | - |
Plasmodium falciparum | D-fructose 6-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, recombinant enzyme, SDS-PAGE | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
Glucose 6-phosphate isomerase | - |
Plasmodium falciparum |
GPI | - |
Plasmodium falciparum |
PGI | - |
Plasmodium falciparum |
PHI | - |
Plasmodium falciparum |
Phosphoglucose isomerase | - |
Plasmodium falciparum |
Phosphohexose isomerase | - |
Plasmodium falciparum |
General Information | Comment | Organism |
---|---|---|
metabolism | PGI is a key enzyme in glycolysis and glycogenesis catalyzing the second step of glycolysis | Plasmodium falciparum |