Literature summary for 5.3.1.9 extracted from

Berrisford, J.M.; Hounslow, A.M.; Akerboom, J.; Hagen, W.R.; Brouns, S.J.; van der Oost, J.; Murray, I.A.; Michael Blackburn, G.; Waltho, J.P.; Rice, D.W.; Baker, P.J.
Evidence supporting a cis-enediol-based mechanism for Pyrococcus furiosus phosphoglucose isomerase (2006), J. Mol. Biol., 358, 1353-1366.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
in presence of bound zinc, substrate D-fructose 6-phosphate and several competitive inhibitors	Pyrococcus furiosus

Inhibitors

Inhibitors	Comment	Organism	Structure
5-phospho-D-arabinonate	competitive	Pyrococcus furiosus
5-phospho-D-arabinonohydroxamate	competitive, stable analogue of putative cis-endiol intermediate	Pyrococcus furiosus
EDTA	100fold excess, complete loss of activity within 10 min. 93% of activity may be recovered by additon of 1000fold excess of Zn2+	Pyrococcus furiosus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.5	-	D-fructose 6-phosphate	50°C	Pyrococcus furiosus

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus furiosus	P83194	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pyrococcus furiosus

Reaction

Reaction	Comment	Organism	Reaction ID
alpha-D-glucose 6-phosphate = beta-D-fructofuranose 6-phosphate	cis-endiol intermediate based mechanism with Glu97 acting as the catalytic base responsible for isomerization	Pyrococcus furiosus	a

Renatured (Commentary)

Renatured (Comment)	Organism
enzyme inactivated by 100fold excess of EDTA can be reactivated by 1000fold excess of of Zn2+	Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 6-phosphate	-	Pyrococcus furiosus	D-glucose 6-phosphate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
8.4	-	D-fructose 6-phosphate	50°C	Pyrococcus furiosus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.005	-	5-phospho-D-arabinonohydroxamate	50°C	Pyrococcus furiosus
0.27	-	5-phospho-D-arabinonate	50°C	Pyrococcus furiosus

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Release 2023.1 (January 2023)