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Literature summary for 5.3.1.9 extracted from

  • Simon, L.M.; Kotorman, M.; Szajani, B.; Boross, L.
    Preparation and characterization of immobilized glucose-phosphate isomerase (1986), Enzyme Microb. Technol., 8, 222-226.
No PubMed abstract available
Search for more literature for 5.3.1.9

General Stability

General Stability Organism
immobilization increases the stability against urea Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 fructose 6-phosphate glucose 6-phosphate, soluble enzyme Oryctolagus cuniculus
0.2
-
 fructose 6-phosphate glucose 6-phosphate, immobilized enzyme Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
 Oryctolagus cuniculus
-

Storage Stability

Storage Stability Organism
8-10°C, storage stability of enzyme immobilized on different carriers, overview Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Glucose 6-phosphate
-
 Oryctolagus cuniculus Fructose 6-phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 immobilized enzyme Oryctolagus cuniculus
50 55 soluble enzyme Oryctolagus cuniculus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 30°C: about 45% of maximal activity of immobilized enzyme, about 35% of maximal activity of soluble enzyme, 60°C: about 60% of maximal activity of immobilized enzyme, about 65% of maximal activity of immobilized enzyme Oryctolagus cuniculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 soluble enzyme, t1/2: 42 min. Immobilized enzyme, t1/2: 82 min Oryctolagus cuniculus
50
-
 soluble enzyme, t1/2: 12 min. Immobilized enzyme, t1/2: 10 min Oryctolagus cuniculus
55
-
 soluble enzyme, t1/2: 2 min. Immobilized enzyme, t1/2: 1 min Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 soluble and immobilized enzyme Oryctolagus cuniculus

pH Range

pH Minimum pH Maximum Comment Organism
6 10 pH 6.0: about 70% of maximal activity of soluble enzyme, about 30% of maximal activity of immobilized enzyme Oryctolagus cuniculus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 immobilized enzyme: no marked change in the first few hours at different pH levels from pH 6.0-9.0, after a longer incubation the highest stability is measured at pH 6.0 Oryctolagus cuniculus
8
-
 highest stability of soluble enzyme Oryctolagus cuniculus